A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector
Joint Authors
Xiong, Ping
Hu, Futao
Gan, Ning
Li, Tianhua
Zheng, Lei
Cao, Yuting
Wang, Ji
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A novel horseradish peroxidase- (HPR-) doped magnetic core-shell Fe3O4@SiO2@Au nanocomposites (Fe-Au MNPs) were employed on immunoassay for the determination of C-reactive protein (CRP) based on a electrochemical quartz crystal microbalance detector (EQCM).
Firstly, the secondary CRP antibody and HRP were both immobilized on the Fe-Au MNPs (Fe-Au MNPs-anti-CRP2/HRP) as a signal tag.
Secondly, the above tag and the primary antibody (anti-CRP1) in the bottom of 96-well microtiter plate were employed to conjugate with a serial of CRP concentrations to produce a sandwich immunocomplex.
Thirdly, the immunocomplex solution was subsequently exposed to 3,3′-diaminobenzidine (DAB) in the presence of H2O2, resulting in an insoluble product.
When the precipitation solution was dripped on EQCM, it can achieve a decrease of frequency of crystal (Δf).
The amount of Δf was proportional to (CRP) from 0.003 to 200 ng mL−1 with a low detection limit of 1 pg mL−1.
Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag.
It also has good specificity and low sample consumption, which is expected to be a benefit for the CRP screening in early diagnosis of cardiovascular disease.
American Psychological Association (APA)
Gan, Ning& Xiong, Ping& Wang, Ji& Li, Tianhua& Hu, Futao& Cao, Yuting…[et al.]. 2013. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-475085
Modern Language Association (MLA)
Gan, Ning…[et al.]. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-475085
American Medical Association (AMA)
Gan, Ning& Xiong, Ping& Wang, Ji& Li, Tianhua& Hu, Futao& Cao, Yuting…[et al.]. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-475085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475085