Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
Joint Authors
Lopes, Fernanda Cortez
Tichota, Deise Michele
Brandelli, Adriano
Corrêa, Ana Paula Folmer
Velho, Renata Voltolini
Pereira, Jamile Queiroz
Daroit, Daniel Joner
Silva, Lucas André Dedavid e
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA.
This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal.
The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h.
These enzymes were partly characterized as serine protease and aspartic protease, respectively.
The effects of feather meal concentration and initial pH on enzyme production were evaluated using a central composite design combined with response surface methodology.
The optimal conditions were determined as pH 5.0 for protease and 7.8 for keratinase and 20 g/L of feather meal, showing that both models were predictive.
Production of keratinases by A.
niger is a less-exploited field that might represent a novel and promising biotechnological application for this microorganism.
American Psychological Association (APA)
Lopes, Fernanda Cortez& Silva, Lucas André Dedavid e& Tichota, Deise Michele& Daroit, Daniel Joner& Velho, Renata Voltolini& Pereira, Jamile Queiroz…[et al.]. 2011. Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger. Enzyme Research،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-475543
Modern Language Association (MLA)
Lopes, Fernanda Cortez…[et al.]. Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger. Enzyme Research No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-475543
American Medical Association (AMA)
Lopes, Fernanda Cortez& Silva, Lucas André Dedavid e& Tichota, Deise Michele& Daroit, Daniel Joner& Velho, Renata Voltolini& Pereira, Jamile Queiroz…[et al.]. Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-475543
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475543