Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells

Joint Authors

Raftery, Mark J.
Mendz, George L.
Okoli, Arinze S.

Source

International Journal of Hepatology

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-07-30

Country of Publication

Egypt

No. of Pages

16

Main Subjects

Medicine

Abstract EN

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide.

Hepatitis B or C infections are the main causes of HCC with hepatitis C being the major risk factor for liver cancer in the developed countries.

Recently, complications with bacteria of the genus Helicobacter have been associated with HCV-induced HCC.

To further understand the mechanisms leading to the development of HCC in the presence of HCV and/or Helicobacter spp., investigation of the differential protein expression in Huh7 cells harbouring HCV-replicon, and replicon cured-Huh7 cells cocultured with H.

bilis was done employing two-dimensional gel electrophoresis and mass spectrometry.

In the transfected-Huh7 cells exposed to sublethal inoculum densities of H.

bilis, 53 different proteins were identified comprising of 28 upregulated and 16 downregulated proteins including 9 potential protein isoforms; in the cured Huh7 cells, 45 different proteins were identified including 33 upregulated, 8 downregulated and, 9 potential protein isoforms.

H.

bilis affected the modulation of proteins involved in different pathways of Huh7-derived cells physiology including proteins involved in the progression from dysplasia to neoplasm.

The result also indicated that the response of the Huh7-derived cells to the presence of H.

bilis depended on whether or not HCV replicon was present.

American Psychological Association (APA)

Okoli, Arinze S.& Raftery, Mark J.& Mendz, George L.. 2012. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-476601

Modern Language Association (MLA)

Okoli, Arinze S.…[et al.]. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-476601

American Medical Association (AMA)

Okoli, Arinze S.& Raftery, Mark J.& Mendz, George L.. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-476601

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-476601