Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells
Joint Authors
Raftery, Mark J.
Mendz, George L.
Okoli, Arinze S.
Source
International Journal of Hepatology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-30
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide.
Hepatitis B or C infections are the main causes of HCC with hepatitis C being the major risk factor for liver cancer in the developed countries.
Recently, complications with bacteria of the genus Helicobacter have been associated with HCV-induced HCC.
To further understand the mechanisms leading to the development of HCC in the presence of HCV and/or Helicobacter spp., investigation of the differential protein expression in Huh7 cells harbouring HCV-replicon, and replicon cured-Huh7 cells cocultured with H.
bilis was done employing two-dimensional gel electrophoresis and mass spectrometry.
In the transfected-Huh7 cells exposed to sublethal inoculum densities of H.
bilis, 53 different proteins were identified comprising of 28 upregulated and 16 downregulated proteins including 9 potential protein isoforms; in the cured Huh7 cells, 45 different proteins were identified including 33 upregulated, 8 downregulated and, 9 potential protein isoforms.
H.
bilis affected the modulation of proteins involved in different pathways of Huh7-derived cells physiology including proteins involved in the progression from dysplasia to neoplasm.
The result also indicated that the response of the Huh7-derived cells to the presence of H.
bilis depended on whether or not HCV replicon was present.
American Psychological Association (APA)
Okoli, Arinze S.& Raftery, Mark J.& Mendz, George L.. 2012. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-476601
Modern Language Association (MLA)
Okoli, Arinze S.…[et al.]. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-476601
American Medical Association (AMA)
Okoli, Arinze S.& Raftery, Mark J.& Mendz, George L.. Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-476601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476601