Optimization on Preparation Condition of Propolis Flavonoids Liposome by Response Surface Methodology and Research of Its Immunoenhancement Activity
Joint Authors
Liu, Xu
Lu, Yu
Yuan, Ju
Liu, Jiaguo
Fan, Yunpeng
Zhao, Xiaojuan
Wang, Deyun
Abula, Saifuding
Hu, Yuanliang
Liu, Cui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study is to prepare propolis flavonoids liposome (PFL) and optimize the preparation condition and to investigate further whether liposome could promote the immunoenhancement activity of propolis flavonoids (PF).
PFL was prepared with ethanol injection method, and the preparation conditions of PFL were optimized with response surface methodology (RSM).
Moreover, the immunoenhancement activity of PFL and PF in vitro was determined.
The result showed that the optimal preparation conditions for PFL by response surface methodology were as follows: ratio of lipid to drug (w/w) 9.6 : 1, ratio of soybean phospholipid to cholesterol (w/w) 8.5 : 1, and speed of injection 0.8 mL·min−1.
Under these conditions, the experimental encapsulation efficiency of PFL was 91.67 ± 0.21%, which was close to the predicted value.
Therefore, the optimized preparation condition is very reliable.
Moreover, the results indicated that PFL could not only significantly promote lymphocytes proliferation singly or synergistically with PHA, but also increase expression level of IL-2 and IFN-γ mRNA.
These indicated that liposome could significantly improve the immunoenhancement activity of PF.
PFL demonstrates the significant immunoenhancement activity, which provides the theoretical basis for the further experiment in vivo.
American Psychological Association (APA)
Yuan, Ju& Lu, Yu& Abula, Saifuding& Hu, Yuanliang& Liu, Jiaguo& Fan, Yunpeng…[et al.]. 2013. Optimization on Preparation Condition of Propolis Flavonoids Liposome by Response Surface Methodology and Research of Its Immunoenhancement Activity. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476987
Modern Language Association (MLA)
Yuan, Ju…[et al.]. Optimization on Preparation Condition of Propolis Flavonoids Liposome by Response Surface Methodology and Research of Its Immunoenhancement Activity. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-476987
American Medical Association (AMA)
Yuan, Ju& Lu, Yu& Abula, Saifuding& Hu, Yuanliang& Liu, Jiaguo& Fan, Yunpeng…[et al.]. Optimization on Preparation Condition of Propolis Flavonoids Liposome by Response Surface Methodology and Research of Its Immunoenhancement Activity. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476987