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Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit
Joint Authors
Premarathne, A. A. A.
Leung, David W. M.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-04
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Aminopeptidase (AP) activity in ripe but firm fruit of Actinidia deliciosa was characterized using L-leucine-p-nitroanilide as a substrate.
The enzyme activity was the highest under alkaline conditions and was thermolabile.
EDTA, 1,10-phenanthroline, iodoacetamide, and Zn2+ had inhibitory effect while a low concentration of dithiothreitol (DTT) had stimulatory effect on kiwifruit AP activity.
However, DTT was not essential for the enzyme activity.
The results obtained indicated that the kiwifruit AP was a thiol-dependent metalloprotease.
Its activity was the highest in the seeds, followed by the core and pericarp tissues of the fruit.
The elution profile of the AP activity from a DEAE-cellulose column suggested that there were at least two AP isozymes in kiwifruit: one unadsorbed and one adsorbed fractions.
It is concluded that useful food-grade aminopeptidases from kiwifruit could be revealed using more specific substrates.
American Psychological Association (APA)
Premarathne, A. A. A.& Leung, David W. M.. 2010. Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit. Enzyme Research،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-477965
Modern Language Association (MLA)
Premarathne, A. A. A.& Leung, David W. M.. Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit. Enzyme Research No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-477965
American Medical Association (AMA)
Premarathne, A. A. A.& Leung, David W. M.. Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-477965
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477965