Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration
Joint Authors
Patel, Mrunali R.
Kartik, Vikani
Patel, Rashmin B.
Mehul, Gopani
Bhaumik, Patel
Source
Chromatography Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
This paper describes validated reverse phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of trihexyphenidyl hydrochloride (THP) and risperidone (RSP) in the pure powder form and in combined tablet dosage form.
The HPLC separation was achieved on a core shell C18 (100 mm length × 4.6 mm, 2.6 μm particle size) using methanol : ammonium acetate buffer 1% (85 : 15 v/v; pH-6.5) as mobile phase and delivered at flow rate of 0.8 mL/min.
The calibration plot showed good linear relationship with r2 = 0.997 ± 0.001 for THP and r2 = 0.998 ± 0.001 for RSP in concentration range of 50–175 μg/mL and 50–175 μg/mL, respectively.
LOD and LOQ were found to be 0.40 and 1.29 μg/mL for THP and 1.24 and 3.92 μg/mL for RSP.
Assay of THP and RSP was found to be 100.16 ± 0.03% and 99.83 ± 0.02%, respectively.
THP and RSP were subjected to different stress conditions (acidic, basic, oxidative, thermal, and photolytic degradation).
The degraded product peaks were well resolved from the pure drug peak.
The method was successfully validated as per the ICH guidelines.
The developed RP-HPLC method was successfully applied for the estimation of THP and RSP in tablet dosage form.
American Psychological Association (APA)
Bhaumik, Patel& Mehul, Gopani& Kartik, Vikani& Patel, Rashmin B.& Patel, Mrunali R.. 2014. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-478415
Modern Language Association (MLA)
Bhaumik, Patel…[et al.]. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-478415
American Medical Association (AMA)
Bhaumik, Patel& Mehul, Gopani& Kartik, Vikani& Patel, Rashmin B.& Patel, Mrunali R.. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-478415
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478415