Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration

Joint Authors

Patel, Mrunali R.
Kartik, Vikani
Patel, Rashmin B.
Mehul, Gopani
Bhaumik, Patel

Source

Chromatography Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-03-19

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Chemistry

Abstract EN

This paper describes validated reverse phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of trihexyphenidyl hydrochloride (THP) and risperidone (RSP) in the pure powder form and in combined tablet dosage form.

The HPLC separation was achieved on a core shell C18 (100 mm length × 4.6 mm, 2.6 μm particle size) using methanol : ammonium acetate buffer 1% (85 : 15 v/v; pH-6.5) as mobile phase and delivered at flow rate of 0.8 mL/min.

The calibration plot showed good linear relationship with r2 = 0.997 ± 0.001 for THP and r2 = 0.998 ± 0.001 for RSP in concentration range of 50–175 μg/mL and 50–175 μg/mL, respectively.

LOD and LOQ were found to be 0.40 and 1.29 μg/mL for THP and 1.24 and 3.92 μg/mL for RSP.

Assay of THP and RSP was found to be 100.16 ± 0.03% and 99.83 ± 0.02%, respectively.

THP and RSP were subjected to different stress conditions (acidic, basic, oxidative, thermal, and photolytic degradation).

The degraded product peaks were well resolved from the pure drug peak.

The method was successfully validated as per the ICH guidelines.

The developed RP-HPLC method was successfully applied for the estimation of THP and RSP in tablet dosage form.

American Psychological Association (APA)

Bhaumik, Patel& Mehul, Gopani& Kartik, Vikani& Patel, Rashmin B.& Patel, Mrunali R.. 2014. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-478415

Modern Language Association (MLA)

Bhaumik, Patel…[et al.]. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-478415

American Medical Association (AMA)

Bhaumik, Patel& Mehul, Gopani& Kartik, Vikani& Patel, Rashmin B.& Patel, Mrunali R.. Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation : Development and Validation Consideration. Chromatography Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-478415

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-478415