Therapeutic Potential of Tacrolimus on Acute Myocardial Infarction in Minipigs : Analysis with Serial Cardiac Magnetic Resonance and Changes at Histological and Protein Levels
Joint Authors
Ko, Sheung-Fat
Lee, Chia-Chang
Lee, Chen-Chang
Chen, Min-Chi
Huang, Chung-Cheng
Sun, Cheuk-Kwan
Ng, Shu-Hang
Yip, Hon-Kan
Sheu, Jiunn-Jye
Leu, Steve
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
This study investigates the therapeutic potential of intracoronary tacrolimus against acute myocardial infarction (AMI) in minipigs with serial cardiac magnetic resonance (CMR) and changes at histological and protein levels.
Twelve minipigs subjected to permanent left anterior descending artery ligation were randomized as tac-treated group (n=6, with intracoronary tacrolimus treatment) and controls (n=6).
CMR with cine and late gadolinium enhancement (LGE) studies were performed on postoperative days 2, 5, and 21.
There were no significant differences in left ventricular function (LVF), contractility, and LGE between the two groups on day 2.
On day 5, the tac-treated group showed a significantly higher ejection fraction, smaller infarct, and lower day-5/day-2 infarct ratio than controls.
On day 21, the controls demonstrated further deterioration of LVF and infarct.
Contrastingly, the tac-treated animals demonstrated preservation of LVF, contractility, significantly smaller infarct, and lower day-21/day-2 infarct ratios compared with those on day 5 and controls.
The in vivo CMR results were correlated with in vitro findings on histology, immunostaining, and Western blotting which revealed significantly less fibrosis, higher vascularities, less CD68+ and CD40+ inflammatory cells, lower expressions of inflammatory (MMP-9, NF-κB, and TNF-α), and apoptotic (Bax, Caspase-3, c-PARP) biomarkers, respectively, in tac-treated AMI minipigs than controls.
American Psychological Association (APA)
Ko, Sheung-Fat& Yip, Hon-Kan& Leu, Steve& Lee, Chen-Chang& Sheu, Jiunn-Jye& Lee, Chia-Chang…[et al.]. 2014. Therapeutic Potential of Tacrolimus on Acute Myocardial Infarction in Minipigs : Analysis with Serial Cardiac Magnetic Resonance and Changes at Histological and Protein Levels. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-478495
Modern Language Association (MLA)
Ko, Sheung-Fat…[et al.]. Therapeutic Potential of Tacrolimus on Acute Myocardial Infarction in Minipigs : Analysis with Serial Cardiac Magnetic Resonance and Changes at Histological and Protein Levels. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-478495
American Medical Association (AMA)
Ko, Sheung-Fat& Yip, Hon-Kan& Leu, Steve& Lee, Chen-Chang& Sheu, Jiunn-Jye& Lee, Chia-Chang…[et al.]. Therapeutic Potential of Tacrolimus on Acute Myocardial Infarction in Minipigs : Analysis with Serial Cardiac Magnetic Resonance and Changes at Histological and Protein Levels. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-478495
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478495