Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase

Joint Authors

Tchorbanov, Bozhidar P.
Grozeva, Lydia
Marinova, Margarita D.

Source

Enzyme Research

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-08-24

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Biology

Abstract EN

Yoghurt strain Lactobacillus LBL-4 cultivated for 8–10 h at pH ~6.0 was investigated as a considerable food-grade source of intracellular aminopeptidase.

Cell-free extract manifesting >200 AP U/l was obtained from cells harvested from 1 L culture media.

Subtilisin-induced hydrolysates of casein, soybean isolate, and Scenedesmus cell protein with degree of hydrolysis 20–22% incubated at 45∘C for 10 h by 10 AP U/g peptides caused an enlarging of DH up to 40–42%, 46–48%, and 38–40% respectively.

The DH increased rapidly during the first 4 h, but gel chromatography studies on BioGel P-2 showed significant changes occurred during 4–10 h of enzyme action when the DH increased gradually.

After the digestion, the remained AP activity can be recovered by ultrafiltration (yield 40–50%).

Scenedesmus protein hydrolysate with DH 20% was inoculated by Lactobacillus LBL-4 cells, and after 72 h cultivation the DH reached 32%.

The protein hydrolysates (DH above 40%) obtained from casein and soybean isolate (high Q value) demonstrated a negligible bitterness while Scenedesmus protein hydrolysates (low Q value) after both treatments were free of bitterness.

American Psychological Association (APA)

Tchorbanov, Bozhidar P.& Marinova, Margarita D.& Grozeva, Lydia. 2011. Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-479732

Modern Language Association (MLA)

Tchorbanov, Bozhidar P.…[et al.]. Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-479732

American Medical Association (AMA)

Tchorbanov, Bozhidar P.& Marinova, Margarita D.& Grozeva, Lydia. Debittering of Protein Hydrolysates by Lactobacillus LBL-4 Aminopeptidase. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-479732

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-479732