Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity of Colletotrichum gloeosporioides
Joint Authors
Abdul Murad, Abdul Munir
Abu Bakar, Farah Diba
Priyatno, Tri Puji
Kamaruddin, Nurhaida
Mahadi, Nor Muhammad
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-01
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The cyclic AMP- (cAMP-) dependent protein kinase A signaling pathway is one of the major signaling pathways responsible for regulation of the morphogenesis and pathogenesis of several pathogenic fungi.
To evaluate the role of this pathway in the plant pathogenic fungus, Colletotrichum gloeosporioides, the gene encoding the catalytic subunit of cAMP-dependent protein kinase A, CgPKAC, was cloned, inactivated, and the mutant was analyzed.
Analysis of the Cgpkac mutant generated via gene replacement showed that the mutants were able to form appressoria; however, their formation was delayed compared to the wild type.
In addition, the mutant conidia underwent bipolar germination after appressoria formation, but no appressoria were generated from the second germ tube.
The mutants also showed reduced ability to adhere to a hydrophobic surface and to degrade lipids localized in the appressoria.
Based on the number of lesions produced during a pathogenicity test, the mutant’s ability to cause disease in healthy mango fruits was reduced, which may be due to failure to penetrate into the fruit.
These findings indicate that cAMP-dependent protein kinase A has an important role in regulating morphogenesis and is required for pathogenicity of C.
gloeosporioides.
American Psychological Association (APA)
Priyatno, Tri Puji& Abu Bakar, Farah Diba& Kamaruddin, Nurhaida& Mahadi, Nor Muhammad& Abdul Murad, Abdul Munir. 2012. Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity of Colletotrichum gloeosporioides. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-480319
Modern Language Association (MLA)
Priyatno, Tri Puji…[et al.]. Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity of Colletotrichum gloeosporioides. The Scientific World Journal No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-480319
American Medical Association (AMA)
Priyatno, Tri Puji& Abu Bakar, Farah Diba& Kamaruddin, Nurhaida& Mahadi, Nor Muhammad& Abdul Murad, Abdul Munir. Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity of Colletotrichum gloeosporioides. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-480319
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480319