A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations
Joint Authors
Pierre, Melanie
Ilaria, Lippi
Grazia, Guidi
Valentina, Meucci
Gloria, Breghi
Source
Journal of Veterinary Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Glomerular filtration rate (GFR) is still a highly underutilized tool in cats because available methods are not easy to be performed in clinical practice.
Iohexol (IOX) has been shown to be a useful and reliable marker of GFR both in animals and in humans.
The aim of the present study was to develop a rapid and reliable method for measuring IOX in feline plasma and to evaluate the accuracy of limited sampling models to establish a low-cost and clinically suitable GFR test.
IOX concentrations were determined by using a new HPLC-UV method.
GFR was assessed as plasma clearance of IOX, which was calculated by dividing dose administered by area under the curve of plasmatic concentration versus time (AUC), and indexed to body weight (BW).
Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour.
The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting.
American Psychological Association (APA)
Valentina, Meucci& Grazia, Guidi& Pierre, Melanie& Gloria, Breghi& Ilaria, Lippi. 2013. A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations. Journal of Veterinary Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-481465
Modern Language Association (MLA)
Valentina, Meucci…[et al.]. A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations. Journal of Veterinary Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-481465
American Medical Association (AMA)
Valentina, Meucci& Grazia, Guidi& Pierre, Melanie& Gloria, Breghi& Ilaria, Lippi. A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations. Journal of Veterinary Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-481465
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481465