Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production
Joint Authors
Lee, Young-Chul
Jeon, Bo Ra
Im, Eun Ju
Park, Ji Young
Jia, Qi
Lee, Joo Young
Son, Young Min
Park, Hwa-Jin
Lee, Dong-Ha
Rhee, Man Hee
Ji, Hyun Dong
Hyun, Eujin
Hong, Mei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A number of reagents that prevent thrombosis have been developed but were found to have serious side effects.
Therefore, we sought to identify complementary and alternative medicinal materials that are safe and have long-term efficacy.
In the present studies, we have assessed the ability of chlorine e6 (CE6) to inhibit ADP-induced aggregation of rat platelets and elucidated the underlying mechanism.
CE6 inhibited platelet aggregation induced by 10 µM ADP in a concentration-dependent manner and decreased intracellular calcium mobilization and granule secretion (i.e., ATP and serotonin release).
Western blotting revealed that CE6 strongly inhibited the phosphorylations of PI3K, Akt, c-Jun N-terminal kinase (JNK), and different mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase 1/2 (ERK1/2) as well as p38-MAPK.
Our study also demonstrated that CE6 significantly elevated intracellular cAMP levels and decreased thromboxane A2 formation in a concentration-dependent manner.
Furthermore, we determined that CE6 initiated the activation of PKA, an effector of cAMP.
Taken together, our findings indicate that CE6 may inhibit ADP-induced platelet activation by elevating cAMP levels and suppressing PI3K/Akt activity.
Finally, these results suggest that CE6 could be developed as therapeutic agent that helps prevent thrombosis and ischemia.
American Psychological Association (APA)
Park, Ji Young& Ji, Hyun Dong& Jeon, Bo Ra& Im, Eun Ju& Son, Young Min& Lee, Joo Young…[et al.]. 2013. Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-481473
Modern Language Association (MLA)
Park, Ji Young…[et al.]. Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-481473
American Medical Association (AMA)
Park, Ji Young& Ji, Hyun Dong& Jeon, Bo Ra& Im, Eun Ju& Son, Young Min& Lee, Joo Young…[et al.]. Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-481473
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481473