Aberrant Frequency of IL-10-Producing B Cells and Its Association with TregTh17 in Adult Primary Immune Thrombocytopenia Patients
Joint Authors
Gao, Song
Zou, Shanhua
Li, Ying
Li, Feng
Chen, Lihang
Hua, Fanli
Cheng, Yunfeng
Zhan, Yanxia
Ji, Lili
Chen, Hao
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Regulatory B cells (Breg) are a distinct B cell subset with immunoregulatory properties.
Pivotal to Breg function is interleukin-10.
This study was to investigate the role of IL-10-producing B cell (B10) and its association with Treg and Th17 subsets in immune thrombocytopenia (ITP) patients.
Methods.
Peripheral blood mononuclear cells from ITP patients and controls were stimulated with PMA, ionomycin, and Brefeldin A.
The frequencies of CD19+IL-10+ B cells, CD3+CD4+IL-17+ Th17 cells, and CD4+CD25hiFoxp3+ Treg cells were analyzed by flow cytometry.
The mRNA expression of Foxp3 and RORγt was detected by real-time quantitative PCR.
Results.
The number of B10 cells was elevated in ITP patients.
After first-line therapies, it remained at high level in patients who achieved complete or partial response but decreased in those who acquired no response.
There was a positive correlation between B10 cells and Tregs in ITP both before and after therapies.
The ratio of Treg/Th17 decreased in ITP, and it strongly correlated with B10 cells.
Conclusions.
The frequency of B10 cells is elevated in ITP and it correlates with both the Tregs counts and the Treg/Th17 ratio.
B10 cells to regulate functional T cell subsets might be impaired in patients with ITP.
American Psychological Association (APA)
Hua, Fanli& Ji, Lili& Zhan, Yanxia& Li, Feng& Zou, Shanhua& Chen, Lihang…[et al.]. 2014. Aberrant Frequency of IL-10-Producing B Cells and Its Association with TregTh17 in Adult Primary Immune Thrombocytopenia Patients. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-481644
Modern Language Association (MLA)
Hua, Fanli…[et al.]. Aberrant Frequency of IL-10-Producing B Cells and Its Association with TregTh17 in Adult Primary Immune Thrombocytopenia Patients. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-481644
American Medical Association (AMA)
Hua, Fanli& Ji, Lili& Zhan, Yanxia& Li, Feng& Zou, Shanhua& Chen, Lihang…[et al.]. Aberrant Frequency of IL-10-Producing B Cells and Its Association with TregTh17 in Adult Primary Immune Thrombocytopenia Patients. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-481644
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481644