Rapid Determination of Ractopamine Residues in Edible Animal Products by Enzyme-Linked Immunosorbent Assay : Development and Investigation of Matrix Effects
Joint Authors
Fang, Li
Fang, GuoZhen
Wang, FengXia
Wang, Shuo
Zhang, Yan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To determine ractopamine residues in animal food products (chicken muscle, pettitoes, pig muscle, and pig liver), we established a rapid direct competitive enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody generated from ractopamine-linker-BSA.
The antibody showed high sensitivity and specificity in phosphate buffer, with an IC50 of 0.6 ng/mL, and the limit of detection was 0.04 ng/mL.
The matrix effect of the samples was easily eliminated by one-step extraction with PBS, without any organic solution or clean-up procedure such as SPE or liquid-liquid extraction, making it a much more simple and rapid method than previously reported ones.
The detection limit in blank samples was 0.2 μg/kg.
To validate this new RAC (ractopamine hydrochloride) ELISA, a RAC-free pig liver sample spiked at three different concentrations was prepared and analyzed by HPLC and ELISA.
The results showed a good correlation between the data of ELISA and HPLC (R2>0.95), which proves that the established ELISA is accurate enough to quantify the residue of RAC in the animal derived foods.
American Psychological Association (APA)
Zhang, Yan& Wang, FengXia& Fang, Li& Wang, Shuo& Fang, GuoZhen. 2009. Rapid Determination of Ractopamine Residues in Edible Animal Products by Enzyme-Linked Immunosorbent Assay : Development and Investigation of Matrix Effects. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-482291
Modern Language Association (MLA)
Zhang, Yan…[et al.]. Rapid Determination of Ractopamine Residues in Edible Animal Products by Enzyme-Linked Immunosorbent Assay : Development and Investigation of Matrix Effects. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-482291
American Medical Association (AMA)
Zhang, Yan& Wang, FengXia& Fang, Li& Wang, Shuo& Fang, GuoZhen. Rapid Determination of Ractopamine Residues in Edible Animal Products by Enzyme-Linked Immunosorbent Assay : Development and Investigation of Matrix Effects. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-482291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482291