BACs-on-Beads Technology : A Reliable Test for Rapid Detection of Aneuploidies and Microdeletions in Prenatal Diagnosis
Joint Authors
Campos-Galindo, Inmaculada
Rubio, Carmen
Serra, Vicente
García-Herrero, Sandra
Olmo, Inés
Simón, Carlos
Martínez-Conejero, José Antonio
Lara, Coral
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The risk of fetal aneuploidies is usually estimated based on high resolution ultrasound combined with biochemical determination of criterion in maternal blood, with invasive procedures offered to the population at risk.
The purpose of this study was to investigate the effectiveness of a new rapid aneuploidy screening test on amniotic fluid (AF) or chorionic villus (CV) samples based on BACs-on-Beads (BoBs) technology and to compare the results with classical karyotyping by Giemsa banding (G-banding) of cultured cells in metaphase as the gold standard technique.
The prenatal-BoBs kit was used to study aneuploidies involving chromosomes 13, 18, 21, X, and Y as well as nine microdeletion syndromes in 321 AF and 43 CV samples.
G-banding of metaphase cultured cells was performed concomitantly for all prenatal samples.
A microarray-based comparative genomic hybridization (aCGH) was also carried out in a subset of samples.
Prenatal-BoBs results were widely confirmed by classical karyotyping.
Only six karyotype findings were not identified by Prenatal-BoBs, all of them due to the known limitations of the technique.
In summary, the BACs-on-Beads technology was an accurate, robust, and efficient method for the rapid diagnosis of common aneuploidies and microdeletion syndromes in prenatal samples.
American Psychological Association (APA)
García-Herrero, Sandra& Campos-Galindo, Inmaculada& Martínez-Conejero, José Antonio& Serra, Vicente& Olmo, Inés& Lara, Coral…[et al.]. 2014. BACs-on-Beads Technology : A Reliable Test for Rapid Detection of Aneuploidies and Microdeletions in Prenatal Diagnosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-483218
Modern Language Association (MLA)
García-Herrero, Sandra…[et al.]. BACs-on-Beads Technology : A Reliable Test for Rapid Detection of Aneuploidies and Microdeletions in Prenatal Diagnosis. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-483218
American Medical Association (AMA)
García-Herrero, Sandra& Campos-Galindo, Inmaculada& Martínez-Conejero, José Antonio& Serra, Vicente& Olmo, Inés& Lara, Coral…[et al.]. BACs-on-Beads Technology : A Reliable Test for Rapid Detection of Aneuploidies and Microdeletions in Prenatal Diagnosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-483218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483218