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Protective Mechanisms of S. lycopersicum Aqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation : In Vitro, Ex Vivo and In Vivo Studies
Joint Authors
Fuentes, Eduardo
Valenzuela, Claudio
Palomo, Iván
Astudillo, Luis
Pereira, Jaime
Alarcón, Marcelo
Pérez, Pablo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The purpose of this research was to investigate mechanisms of antiplatelet action of bioactive principle from S.
lycopersicum.
Aqueous fraction had a high content of nucleosides (adenosine, guanosine, and adenosine 5′-monophosphate) by HPLC analysis.
Also aqueous fraction presented flavonoids content.
Aqueous fraction inhibited platelet activation by 15 ± 6% (P<0.05).
Fully spread of human platelets on collagen in the presence of aqueous fraction was inhibited from 15 ± 1 to 9 ± 1 μm2 (P<0.001).
After incubation of whole blood with aqueous fraction, the platelet coverage was inhibited by 55 ± 12% (P<0.001).
Platelet ATP secretion and aggregation were significantly inhibited by the aqueous fraction.
At the same concentrations that aqueous fraction inhibits platelet aggregation, levels of sCD40L significantly decreased and the intraplatelet cAMP levels increased.
In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the effect of aqueous fraction toward ADP-induced platelet aggregation and intraplatelet level of cAMP.
Platelet aggregation ex vivo (human study) and thrombosis formation in vivo (murine model) were inhibited by aqueous fraction.
Finally, aqueous fraction may be used as a functional ingredient adding antiplatelet activities (nucleosides and flavonoids) to processed foods.
American Psychological Association (APA)
Fuentes, Eduardo& Pereira, Jaime& Alarcón, Marcelo& Valenzuela, Claudio& Pérez, Pablo& Astudillo, Luis…[et al.]. 2013. Protective Mechanisms of S. lycopersicum Aqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation : In Vitro, Ex Vivo and In Vivo Studies. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-484878
Modern Language Association (MLA)
Fuentes, Eduardo…[et al.]. Protective Mechanisms of S. lycopersicum Aqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation : In Vitro, Ex Vivo and In Vivo Studies. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-484878
American Medical Association (AMA)
Fuentes, Eduardo& Pereira, Jaime& Alarcón, Marcelo& Valenzuela, Claudio& Pérez, Pablo& Astudillo, Luis…[et al.]. Protective Mechanisms of S. lycopersicum Aqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation : In Vitro, Ex Vivo and In Vivo Studies. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-484878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484878