Both IL-1β and TNF-α Regulate NGAL Expression in Polymorphonuclear Granulocytes of Chronic Hemodialysis Patients
Joint Authors
Arena, Adriana
Iannello, Daniela
Buemi, Michele
Gazzara, Domenica
Lacquaniti, Antonio
Calapai, Maria
Bolignano, Davide
Bisignano, Carlo
Stassi, Giovanna
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
NGAL is involved in modulation of the inflammatory response and is found in the sera of uremic patients.
We investigated whether hemodiafiltration (HDF) could influence the ability of polymorphonuclear granulocytes (PMGs) to release NGAL.
The involvement of interleukin- (IL-)1β and tumor necrosis factor- (TNF-)α on NGAL release was evaluated.
Methods.
We studied end-stage renal disease (ESRD) patients at the start of dialysis (Pre-HDF) and at the end of treatment (Post-HDF) and 18 healthy subjects (HSs).
Peripheral venous blood was taken from HDF patients at the start of dialysis and at the end of treatment.
Results.
PMGs obtained from ESRD patients were hyporesponsive to LPS treatment, with respect to PMG from HS.
IL-1β and TNF-α produced by PMG from post-HDF patients were higher than those obtained by PMG from pre-HDF.
Neutralization of IL-1β, but not of TNF-α, determined a clear-cut production of NGAL in PMG from healthy donors.
On the contrary, specific induction of NGAL in PMG from uremic patients was dependent on the presence in supernatants of IL-1β and TNF-α.
Conclusion.
Our data demonstrate that in PMG from healthy subjects, NGAL production was supported solely by IL-1β, whereas in PMG from HDF patients, NGAL production was supported by IL-1β, TNF-α.
American Psychological Association (APA)
Arena, Adriana& Stassi, Giovanna& Iannello, Daniela& Gazzara, Domenica& Calapai, Maria& Bisignano, Carlo…[et al.]. 2011. Both IL-1β and TNF-α Regulate NGAL Expression in Polymorphonuclear Granulocytes of Chronic Hemodialysis Patients. Mediators of Inflammation،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-485183
Modern Language Association (MLA)
Arena, Adriana…[et al.]. Both IL-1β and TNF-α Regulate NGAL Expression in Polymorphonuclear Granulocytes of Chronic Hemodialysis Patients. Mediators of Inflammation No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-485183
American Medical Association (AMA)
Arena, Adriana& Stassi, Giovanna& Iannello, Daniela& Gazzara, Domenica& Calapai, Maria& Bisignano, Carlo…[et al.]. Both IL-1β and TNF-α Regulate NGAL Expression in Polymorphonuclear Granulocytes of Chronic Hemodialysis Patients. Mediators of Inflammation. 2011. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-485183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485183