Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia : A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy

Joint Authors

Das, Prosun
Chaklader, Malay
Dutta, Ranjan Kumar
Pereira, Jacintha Archana
Law, Sujata
Chaudhuri, Samaresh
Chatterjee, Sumanta
Basak, Pratima
Das, Madhurima

Source

Stem Cells International

Issue

Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2009-10-28

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Natural & Life Sciences (Multidisciplinary)

Abstract EN

Self-renewing Hematopoietic Stem Cells (HSCs) are responsible for reconstitution of all blood cell lineages.

Sca-1 is the “stem cell antigen” marker used to identify the primitive murine HSC population, the expression of which decreases upon differentiation to other mature cell types.

Sca-1+ HSCs maintain the bone marrow stem cell pool throughout the life.

Aplastic anemia is a disease considered to involve primary stem cell deficiency and is characterized by severe pancytopenia and a decline in healthy blood cell generation system.

Studies conducted in our laboratory revealed that the primitive Sca-1+ BM-HSCs (bone marrow hematopoietic stem cell) are significantly affected in experimental Aplastic animals pretreated with chemotherapeutic drugs (Busulfan and Cyclophosphamide) and there is increased Caspase-3 activity with consecutive high Annexin-V positivity leading to premature apoptosis in the bone marrow hematopoietic stem cell population in Aplastic condition.

The Sca-1bright, that is, “more primitive” BM-HSC population was more affected than the “less primitive” BM-HSC Sca-1dim population.

The decreased cell population and the receptor expression were directly associated with an empty and deranged marrow microenvironment, which is evident from scanning electron microscopy (SEM).

The above experimental evidences hint toward the manipulation of receptor expression for the benefit of cytotherapy by primitive stem cell population in Aplastic anemia cases.

American Psychological Association (APA)

Chatterjee, Sumanta& Basak, Pratima& Das, Prosun& Das, Madhurima& Pereira, Jacintha Archana& Dutta, Ranjan Kumar…[et al.]. 2009. Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia : A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy. Stem Cells International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-485238

Modern Language Association (MLA)

Chatterjee, Sumanta…[et al.]. Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia : A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy. Stem Cells International No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-485238

American Medical Association (AMA)

Chatterjee, Sumanta& Basak, Pratima& Das, Prosun& Das, Madhurima& Pereira, Jacintha Archana& Dutta, Ranjan Kumar…[et al.]. Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia : A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy. Stem Cells International. 2009. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-485238

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-485238