The Role of Glucocorticoid Receptors in Dexamethasone-Induced Apoptosis of Neuroprogenitor Cells in the Hippocampus of Rat Pups
Joint Authors
Sze, Chun-I
Hsieh, Ting-Hui
Kuo, Yu Min
Lin, Yung-Chieh
Lin, Chyi-Her
Lin, Yuh-Jyh
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Dexamethasone (Dex) has been used to reduce inflammation in preterm infants with assistive ventilation and to prevent chronic lung diseases.
However, Dex treatment results in adverse effects on the brain.
Since the hippocampus contains a high density of glucocorticoid receptors (GCRs), we hypothesized that Dex affects neurogenesis in the hippocampus through inflammatory mediators.
Methods.
Albino Wistar rat pups first received a single dose of Dex (0.5 mg/kg) on postnatal day 1 (P1) and were sacrificed on P2, P3, P5, and P7.
One group of Dex-treated pups (Dex-treated D1D2) was given mifepristone (RU486, a GCR antagonist) on P1 and sacrificed on P2.
Hippocampi were isolated for western blot analysis, TUNEL, cleaved-caspase 3 staining for cell counts, and morphological assessment.
Control pups received normal saline (NS).
Results.
Dex reduced the developmental gain in body weight, but had no effect on brain weight.
In the Dex-treated D1D2 group, apoptotic cells increased in number based on TUNEL and cleaved-caspase 3 staining.
Most of the apoptotic cells expressed the neural progenitor cell marker nestin.
Dex-induced apoptosis in P1 pups was markedly reduced (60%) by pretreatment with RU486, indicating the involvement of GCRs.
Conclusion.
Early administration of Dex results in apoptosis of neural progenitor cells in the hippocampus and this is mediated through GCRs.
American Psychological Association (APA)
Sze, Chun-I& Lin, Yung-Chieh& Lin, Yuh-Jyh& Hsieh, Ting-Hui& Kuo, Yu Min& Lin, Chyi-Her. 2013. The Role of Glucocorticoid Receptors in Dexamethasone-Induced Apoptosis of Neuroprogenitor Cells in the Hippocampus of Rat Pups. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-486347
Modern Language Association (MLA)
Sze, Chun-I…[et al.]. The Role of Glucocorticoid Receptors in Dexamethasone-Induced Apoptosis of Neuroprogenitor Cells in the Hippocampus of Rat Pups. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-486347
American Medical Association (AMA)
Sze, Chun-I& Lin, Yung-Chieh& Lin, Yuh-Jyh& Hsieh, Ting-Hui& Kuo, Yu Min& Lin, Chyi-Her. The Role of Glucocorticoid Receptors in Dexamethasone-Induced Apoptosis of Neuroprogenitor Cells in the Hippocampus of Rat Pups. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-486347
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486347