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Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions
Joint Authors
Cortez, Erika
Carvalho, Laís
Stumbo, Ana Carolina
Sichieri, Rosely
Bernardo, Amélia F.
Neves, Fabiana A.
Moura, Anibal S.
Garcia-Souza, Érica
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Mitochondria are central coordinators of energy metabolism, and changes of their physiology have long been associated with metabolic disorders.
Thus, observations of energy dynamics in different cell types are of utmost importance.
Therefore, tools with quick and easy handling are needed for consistent evaluations of such interventions.
In this paper, our main hypothesis is that during different nutritional situations lymphocytes mitochondrial physiology could be associated with the metabolism of other cell types, such as cardiomyocytes, and consequently be used as metabolic biomarker.
Blood lymphocytes and heart muscle fibers were obtained from both fed and 24 h-fasted mice, and mitochondrial analysis was assessed by high-resolution respirometry and western blotting.
Carbohydrate-linked oxidation and fatty acid oxidation were significantly higher after fasting.
Carnitine palmitoil transferase 1 and uncouple protein 2 contents were increased in the fasted group, while the glucose transporters 1 and 4 and the ratio phosphorylated AMP-activated protein kinase/AMPK did not change between groups.
In summary, under a nutritional status modification, mitochondria demonstrated earlier adaptive capacity than other metabolic sensors such as glucose transporters and AMPK, suggesting the accuracy of mitochondria physiology of lymphocytes as biomarker for metabolic changes.
American Psychological Association (APA)
Cortez, Erika& Neves, Fabiana A.& Bernardo, Amélia F.& Stumbo, Ana Carolina& Carvalho, Laís& Garcia-Souza, Érica…[et al.]. 2012. Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-486470
Modern Language Association (MLA)
Cortez, Erika…[et al.]. Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-486470
American Medical Association (AMA)
Cortez, Erika& Neves, Fabiana A.& Bernardo, Amélia F.& Stumbo, Ana Carolina& Carvalho, Laís& Garcia-Souza, Érica…[et al.]. Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-486470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486470