Systemic Revealing Pharmacological Signalling Pathway Networks in the Hippocampus of Ischaemia-Reperfusion Mice Treated with Baicalin

Abstract EN

Background.

Baicalin (BA) exhibits ill understood neuroprotective, anti-inflammatory, and antioxidative effects in brain injury.

Objective.

To identify the differential network pathways associated with BA-related biological effects.

Methods.

MCAO-induced mice received BA 5 mg/Kg (BA group).

Controls received vehicle only.

Following ischaemia-reperfusion, ArrayTrack analysed the whole genome microarray of hippocampal genes, and MetaCore analysed differentially expressed genes.

Results.

Four reversing pathways were common to BA and controls, but only 6 were in the top 10 for BA.

Three of the top 5 signalling pathways in controls were not observed in BA.

BA treatment made absent 3 pathways of the top 5 signalling pathways from the top 5 in controls.

There were 2 reversing pathways between controls and BA that showed altered gene expression.

Controls had 6 networks associated with cerebral ischaemia.

After BA treatment, 9 networks were associated with cerebral ischaemia.

Enrichment analysis identified 10 significant biological processes in BA and controls.

Of the 10 most significant molecular functions, 7 were common to BA and controls, and only 3 occurred in BA.

BA and controls had 7 significant cellular components.

Conclusions.

This study showed that the clinical effectiveness of BA was based on the complementary effects of multiple pathways and networks.