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Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus
Joint Authors
Hettihewa, Menik
Nair, Sandhya V. G.
Rupasinghe, H. P. Vasantha
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Costus speciosus is a medicinal plant commonly known as wild ginger distributed in South and Southeast Asian countries.
Leaves of this plant are used for ayurvedic treatment regimes in malignancies and mental illness.
Rhizome extract from the plant is used to treat malignancies, pneumonia, urinary disorders, jaundice, rheumatism, and diabetes.
The goal of this study was to investigate the effects of methanol extract of leaves of C.
speciosus on the growth of human hepatocellular carcinoma (HepG2) cells and understand possible mechanisms of its action.
Viability of HepG2 cells were measured by MTS assay after 24 h and 48 h treatment with extracts of 1, 10, 50, 100, and 200 μg/mL concentrations.
Cell cycle analysis and apoptosis were evaluated by flow cytometry and caspase-3 induction.
HepG2 cells treated with 100 μg/mL methanol leaf extract for 24 h displayed a significant reduction in cell viability (P≤0.05).
The methanol extract perturbed cell cycle progression, modulated cell cycle and regulated, signal molecules were involved in induction of apoptosis in HepG2 cells.
Our findings indicate that phytochemicals of leaves of C.
speciosus shows potential for natural therapeutic product development for hepatocellular carcinoma.
This is the first report to demonstrate in vitro anticancer activity of leaf extract of C.
speciosus in relation to liver cancer.
American Psychological Association (APA)
Nair, Sandhya V. G.& Hettihewa, Menik& Rupasinghe, H. P. Vasantha. 2014. Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-487101
Modern Language Association (MLA)
Nair, Sandhya V. G.…[et al.]. Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-487101
American Medical Association (AMA)
Nair, Sandhya V. G.& Hettihewa, Menik& Rupasinghe, H. P. Vasantha. Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-487101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487101