Association between P2X7 Receptor Polymorphisms and Bone Status in Mice
Joint Authors
Syberg, Susanne
Jensen, Jens-Erik Beck
Schwarz, Peter
Petersen, Solveig
Teilmann, Jenni
Steinberg, Thomas H.
Jorgensen, Niklas Rye
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Macrophages from mouse strains with the naturally occurring mutation P451L in the purinergic receptor P2X7 have impaired responses to agonists (1).
Because P2X7 receptors are expressed in bone cells and are implicated in bone physiology, we asked whether strains with the P451L mutation have a different bone phenotype.
By sequencing the most common strains of inbred mice, we found that only a few strains (BALB, NOD, NZW, and 129) were harboring the wild allelic version of the mutation (P451) in the gene for the purinergic receptor P2X7.
The strains were compared by means of dual energy X-ray absorptiometry (DXA), bone markers, and three-point bending.
Cultured osteoclasts were used in the ATP-induced pore formation assay.
We found that strains with the P451 allele (BALB/cJ and 129X1/SvJ) had stronger femurs and higher levels of the bone resorption marker C-telopeptide collagen (CTX) compared to C57Bl/6 (B6) and DBA/2J mice.
In strains with the 451L allele, pore-formation activity in osteoclasts in vitro was lower after application of ATP.
In conclusion, two strains with the 451L allele of the naturally occurring mutation P451L, have weaker bones and lower levels of CTX, suggesting lower resorption levels in these animals, which could be related to the decreased ATP-induced pore formation observed in vitro.
The importance of these findings for the interpretation of the earlier reported effects of P2X7 in mice is discussed, along with strategies in developing a murine model for testing the therapeutic effects of P2X7 agonists and antagonists upon postmenopausal osteoporosis.
American Psychological Association (APA)
Syberg, Susanne& Schwarz, Peter& Petersen, Solveig& Steinberg, Thomas H.& Jensen, Jens-Erik Beck& Teilmann, Jenni…[et al.]. 2012. Association between P2X7 Receptor Polymorphisms and Bone Status in Mice. Journal of Osteoporosis،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-487184
Modern Language Association (MLA)
Syberg, Susanne…[et al.]. Association between P2X7 Receptor Polymorphisms and Bone Status in Mice. Journal of Osteoporosis No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-487184
American Medical Association (AMA)
Syberg, Susanne& Schwarz, Peter& Petersen, Solveig& Steinberg, Thomas H.& Jensen, Jens-Erik Beck& Teilmann, Jenni…[et al.]. Association between P2X7 Receptor Polymorphisms and Bone Status in Mice. Journal of Osteoporosis. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-487184
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487184