PPI Network Analysis of mRNA Expression Profile of Ezrin Knockdown in Esophageal Squamous Cell Carcinoma
Joint Authors
Zhang, Pixian
Xu, Liyan
Li, Enmin
Wu, Jianyi
Xie, Jianjun
Wu, Bingli
Du, Zepeng
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ezrin, coding protein EZR which cross-links actin filaments, overexpresses and involves invasion, metastasis, and poor prognosis in various cancers including esophageal squamous cell carcinoma (ESCC).
In our previous study, Ezrin was knock down and analyzed by mRNA expression profile which has not been fully mined.
In this study, we applied protein-protein interactions (PPI) network knowledge and methods to explore our understanding of these differentially expressed genes (DEGs).
PPI subnetworks showed that hundreds of DEGs interact with thousands of other proteins.
Subcellular localization analyses found that the DEGs and their directly or indirectly interacting proteins distribute in multiple layers, which was applied to analyze the shortest paths between EZR and other DEGs.
Gene ontology annotation generated a functional annotation map and found hundreds of significant terms, especially those associated with cytoskeleton organization of Ezrin protein, such as “cytoskeleton organization,” “regulation of actin filament-based process,” and “regulation of actin cytoskeleton organization.” The algorithm of Random Walk with Restart was applied to prioritize the DEGs and identified several cancer related DEGs ranked closest to EZR.
These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile of Ezrin knockdown for future examination of the roles and mechanisms of Ezrin.
American Psychological Association (APA)
Wu, Bingli& Xie, Jianjun& Du, Zepeng& Wu, Jianyi& Zhang, Pixian& Xu, Liyan…[et al.]. 2014. PPI Network Analysis of mRNA Expression Profile of Ezrin Knockdown in Esophageal Squamous Cell Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-488379
Modern Language Association (MLA)
Wu, Bingli…[et al.]. PPI Network Analysis of mRNA Expression Profile of Ezrin Knockdown in Esophageal Squamous Cell Carcinoma. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-488379
American Medical Association (AMA)
Wu, Bingli& Xie, Jianjun& Du, Zepeng& Wu, Jianyi& Zhang, Pixian& Xu, Liyan…[et al.]. PPI Network Analysis of mRNA Expression Profile of Ezrin Knockdown in Esophageal Squamous Cell Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-488379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488379