Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation
Joint Authors
de Oliveira, Pollyanna Francielli
Moreno, Gabriela de Padua
Rodrigues, Marina Rezende
Berretta, Andresa Aparecida
Barizon, Edna Aparecida
Costa-Machado, Ana Rita de Mello
Rocha, Bruno Alves
Martins, Sabrina de Paula Lima
Bueno, Paula Carolina Pires
Rodrigues, Andresa Piacezzi Nascimento
Ferreira, Nathália Ursoli
Campos, Jacqueline Costa Lima
Acésio, Nathália de Oliveira
Vaz, Mirela Mara de Oliveira Lima Leite
Tavares, Denise Crispim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Since the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties.
However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE).
Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE.
In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed.
We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays.
We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition.
The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE.
Finally, PWE was shown to be safe using micronucleus assays.
American Psychological Association (APA)
Rocha, Bruno Alves& Bueno, Paula Carolina Pires& Vaz, Mirela Mara de Oliveira Lima Leite& Rodrigues, Andresa Piacezzi Nascimento& Ferreira, Nathália Ursoli& Moreno, Gabriela de Padua…[et al.]. 2013. Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-489110
Modern Language Association (MLA)
Rocha, Bruno Alves…[et al.]. Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-489110
American Medical Association (AMA)
Rocha, Bruno Alves& Bueno, Paula Carolina Pires& Vaz, Mirela Mara de Oliveira Lima Leite& Rodrigues, Andresa Piacezzi Nascimento& Ferreira, Nathália Ursoli& Moreno, Gabriela de Padua…[et al.]. Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-489110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489110