Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels
Joint Authors
Wang, Chen
Xie, Li
Li, Bin
Poyton, Robert O.
Li, Tianlin
Lu, Shu-Ping
Castello, Pablo R.
Easlon, Erin
Tsang, Felicia
Kato, Michiko
Skinner, Craig J.
Lin, Su-Ju
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear.
Recent studies suggest that CR-induced mitochondrial activity is associated with nitric oxide (NO) production.
To understand the role of mitochondria in CR, we identify and study Saccharomyces cerevisiae mutants with increased NO levels as potential CR mimics.
Analysis of the top 17 mutants demonstrates a correlation between increased NO, mitochondrial respiration, and longevity.
Interestingly, treating yeast with NO donors such as GSNO (S-nitrosoglutathione) is sufficient to partially mimic CR to extend lifespan.
CR-increased NO is largely dependent on mitochondrial electron transport and cytochrome c oxidase (COX).
Although COX normally produces NO under hypoxic conditions, CR-treated yeast cells are able to produce NO under normoxic conditions.
Our results suggest that CR may derepress some hypoxic genes for mitochondrial proteins that function to promote the production of NO and the extension of lifespan.
American Psychological Association (APA)
Li, Bin& Skinner, Craig J.& Castello, Pablo R.& Kato, Michiko& Easlon, Erin& Xie, Li…[et al.]. 2011. Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels. Journal of Aging Research،Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-489374
Modern Language Association (MLA)
Li, Bin…[et al.]. Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels. Journal of Aging Research No. 2011 (2011), pp.1-16.
https://search.emarefa.net/detail/BIM-489374
American Medical Association (AMA)
Li, Bin& Skinner, Craig J.& Castello, Pablo R.& Kato, Michiko& Easlon, Erin& Xie, Li…[et al.]. Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels. Journal of Aging Research. 2011. Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-489374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489374