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Inhibition of Nuclear Nox4 Activity by Plumbagin : Effect on Proliferative Capacity in Human Amniotic Stem Cells
Joint Authors
Bertoni, Laura
Zavatti, Manuela
Beretti, Francesca
Resca, Elisa
Maraldi, Tullia
La Sala, Giovanni B.
Guida, Marianna
De Pol, Anto
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Human amniotic fluid stem cells (AFSC) with multilineage differentiation potential are novel source for cell therapy.
However, in vitro expansion leads to senescence affecting differentiation and proliferative capacities.
Reactive oxygen species (ROS) have been involved in the regulation of stem cell pluripotency, proliferation, and differentiation.
Redox-regulated signal transduction is coordinated by spatially controlled production of ROS within subcellular compartments.
NAD(P)H oxidase family, in particular Nox4, has been known to produce ROS in the nucleus; however, the mechanisms and the meaning of this function remain largely unknown.
In the present study, we show that Nox4 nuclear expression (nNox4) increases during culture passages up to cell cycle arrest and the serum starvation causes the same effect.
With the decrease of Nox4 activity, obtained with plumbagin, a decline of nuclear ROS production and of DNA damage occurs.
Moreover, plumbagin exposure reduces the binding between nNox4 and nucleoskeleton components, as Matrin 3.
The same effect was observed also for the binding with phospho-ERK, although nuclear ERK and P-ERK are unchanged.
Taken together, we suggest that nNox4 regulation may have important pathophysiologic effects in stem cell proliferation through modulation of nuclear signaling and DNA damage.
American Psychological Association (APA)
Guida, Marianna& Maraldi, Tullia& Resca, Elisa& Beretti, Francesca& Zavatti, Manuela& Bertoni, Laura…[et al.]. 2013. Inhibition of Nuclear Nox4 Activity by Plumbagin : Effect on Proliferative Capacity in Human Amniotic Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490013
Modern Language Association (MLA)
Guida, Marianna…[et al.]. Inhibition of Nuclear Nox4 Activity by Plumbagin : Effect on Proliferative Capacity in Human Amniotic Stem Cells. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-490013
American Medical Association (AMA)
Guida, Marianna& Maraldi, Tullia& Resca, Elisa& Beretti, Francesca& Zavatti, Manuela& Bertoni, Laura…[et al.]. Inhibition of Nuclear Nox4 Activity by Plumbagin : Effect on Proliferative Capacity in Human Amniotic Stem Cells. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490013