Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata
Joint Authors
Lai, Chih-Ho
Lin, Chun-Jung
Tzeng, Yew-Min
Hsu, Ping-Ning
Rao, Yerra Koteswara
Feng, Chun-Lung
Hung, Chiu-Lien
Tzeng, David T. W.
Lane, Hsien-Yuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The bacterial pathogen Helicobacter pylori (Hp) is the leading risk factor for the development of gastric cancer.
Hp virulence factor, cytotoxin-associated gene A (CagA) interacted with cholesterol-enriched microdomains and leads to induction of inflammation in gastric epithelial cells (AGS).
In this study, we identified a triterpenoid methylantcinate B (MAB) from the medicinal mushroom Antrodia camphoratawhich inhibited the translocation and phosphorylation of CagA and caused a reduction in hummingbird phenotype in HP-infected AGS cells.
Additionally, MAB suppressed the Hp-induced inflammatory response by attenuation of NF-κB activation, translocation of p65 NF-κB, and phosphorylation of IκB-α, indicating that MAB modulates CagA-mediated signaling pathway.
Additionally, MAB also suppressed the IL-8 luciferase activity and its secretion in HP-infected AGS cells.
On the other hand, molecular structure simulations revealed that MAB interacts with CagA similarly to that of cholesterol.
Moreover, binding of cholesterol to the immobilized CagA was inhibited by increased levels of MAB.
Our results demonstrate that MAB is the first natural triterpenoid which competes with cholesterol bound to CagA leading to attenuation of Hp-induced pathogenesis of epithelial cells.
Thus, this study indicates that MAB may have a scope to develop as a therapeutic candidate against Hp CagA-induced inflammation.
American Psychological Association (APA)
Lin, Chun-Jung& Rao, Yerra Koteswara& Hung, Chiu-Lien& Feng, Chun-Lung& Lane, Hsien-Yuan& Tzeng, David T. W.…[et al.]. 2013. Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490160
Modern Language Association (MLA)
Lin, Chun-Jung…[et al.]. Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-490160
American Medical Association (AMA)
Lin, Chun-Jung& Rao, Yerra Koteswara& Hung, Chiu-Lien& Feng, Chun-Lung& Lane, Hsien-Yuan& Tzeng, David T. W.…[et al.]. Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490160
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490160