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Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study
Joint Authors
Patel, Bharat G.
Bhatt, Kashyap K.
Patel, Mrunali R.
Patel, Rashmin B.
Source
Chromatography Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Carbamazepine.
Carbamazepine was chromatographed on silica gel 60 F254 TLC plate using ethyl acetate-toluene-methanol (5.0 + 4.0 + 1.0 v/v/v) as mobile phase.
Carbamazepine was quantified by densitometric analysis at 285 nm.
The method was found to give compact spots for the drug (Rf=0.47 ± 0.01).
The linear regression analysis data for the calibration plots showed good linear relationship with r2=.9995 in the concentration range 100–600 ng/spot.
The method was validated for precision, recovery, repeatability, and robustness as per the International Conference on Harmonization guidelines.
The minimum detectable amount was found to be 16.7 ng/spot, whereas the limit of quantitation was found to be 50.44 ng/spot.
Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Carbamazepine.
The method was successfully employed for the estimation of equilibrium solubility, quantification of Carbamazepine as a bulk drug, in commercially available preparation, and in-house developed mucoadhesive microemulsion formulations and solution.
American Psychological Association (APA)
Patel, Rashmin B.& Patel, Mrunali R.& Bhatt, Kashyap K.& Patel, Bharat G.. 2010. Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study. Chromatography Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-490349
Modern Language Association (MLA)
Patel, Rashmin B.…[et al.]. Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study. Chromatography Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-490349
American Medical Association (AMA)
Patel, Rashmin B.& Patel, Mrunali R.& Bhatt, Kashyap K.& Patel, Bharat G.. Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study. Chromatography Research International. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-490349
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490349