Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model
Joint Authors
Bonamin, Leoni Villano
de Santana, Fabiana Rodrigues
Osugui, Lika
Morante, Graziela
Mariano, Mario
Hurtado, Elizabeth Cristina Perez
Zalla Neto, Ruggero
Popi, Ana Flavia
Cardoso, Thayná Neves
Sato, Cesar
Coelho, Cideli de Paula
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model.
Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated.
The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry.
During all experimental periods, thymulin 5CH and vehicle (control) were given ad libitum to mice, diluted into the drinking water (1.6×10−17 M).
After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells (P=0.0001) in the lesion, in relation to young phagocytes.
After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (P=0.0001).
Simultaneously, the score of infected phagocytes in the lesion decreased (P=0.001), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (P=0.0001).
No difference was seen on the CD25+ Treg cells.
The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation.
American Psychological Association (APA)
Bonamin, Leoni Villano& Sato, Cesar& Zalla Neto, Ruggero& Morante, Graziela& Cardoso, Thayná Neves& de Santana, Fabiana Rodrigues…[et al.]. 2013. Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-490506
Modern Language Association (MLA)
Bonamin, Leoni Villano…[et al.]. Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-490506
American Medical Association (AMA)
Bonamin, Leoni Villano& Sato, Cesar& Zalla Neto, Ruggero& Morante, Graziela& Cardoso, Thayná Neves& de Santana, Fabiana Rodrigues…[et al.]. Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-490506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490506