Amyloid Beta Annular Protofibrils in Cell Processes and Synapses Accumulate with Aging and Alzheimer-Associated Genetic Modification
Joint Authors
Kokubo, Hideko
Glabe, Charles G.
Saido, Takaomi C.
Staufenbiel, Matthias
Kayed, Rakez
Yamaguchi, Haruyasu
Iwata, Nobuhisa
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Amyloid β (Aβ) annular protofibrils (APFs) have been described where the structure is related to that of β barrel pore-forming bacterial toxins and exhibits cellular toxicity.
To investigate the relationship of Aβ APFs to disease and their ultrastructural localization in brain tissue, we conducted a pre-embedding immunoelectron microscopic study using anti-annular protofibril antiserum.
We examined brain tissues of young- and old-aged amyloid precursor protein transgenic mice (APP23), neprilysin knockout APP23 mice, and nontransgenic littermates.
αAPF-immunoreactions tended to be found (1) on plasma membranes and vesicles inside of cell processes, but not on amyloid fibrils, (2) with higher density due to aging, APP transgene, and neprilysin deficiency, and (3) with higher positive rate at synaptic compartments in aged APP23, especially in neprilysin knockout APP23 mice.
These findings imply that APFs are distinct from amyloid fibrils, interact with biological membranes, and might be related to synaptic dysfunction in Alzheimer model mouse brains.
American Psychological Association (APA)
Kokubo, Hideko& Kayed, Rakez& Glabe, Charles G.& Staufenbiel, Matthias& Saido, Takaomi C.& Iwata, Nobuhisa…[et al.]. 2009. Amyloid Beta Annular Protofibrils in Cell Processes and Synapses Accumulate with Aging and Alzheimer-Associated Genetic Modification. International Journal of Alzheimer's Disease،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-490712
Modern Language Association (MLA)
Kokubo, Hideko…[et al.]. Amyloid Beta Annular Protofibrils in Cell Processes and Synapses Accumulate with Aging and Alzheimer-Associated Genetic Modification. International Journal of Alzheimer's Disease No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-490712
American Medical Association (AMA)
Kokubo, Hideko& Kayed, Rakez& Glabe, Charles G.& Staufenbiel, Matthias& Saido, Takaomi C.& Iwata, Nobuhisa…[et al.]. Amyloid Beta Annular Protofibrils in Cell Processes and Synapses Accumulate with Aging and Alzheimer-Associated Genetic Modification. International Journal of Alzheimer's Disease. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-490712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490712