Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice
Joint Authors
Lu, Jie
Lin, Chunlei
Wang, Fan
Shen, Miao
Xu, Xuanfu
Cheng, Ping
He, Lei
Zhou, Ying-Qun
Guo, Chuan-Yong
Wang, Chengfen
Xu, Ling
Weiqi, Dai
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-01
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Objective.
Necrostatin-1 (Nec-1) inhibits receptor-interacting protein 1 (RIP1) kinase and programmed necrosis.
This study was designed to examine the protective effects and mechanisms of Nec-1 in concanavalin A- (ConA-) induced hepatitis in mice.
Methods.
C57BL/6 mice were exposed to ConA via tail vein injection and injected intraperitoneally with Nec-1 or vehicle.
Levels of serum liver enzymes and histopathology were determined.
Levels of inflammatory cytokines with ConA-induced hepatitis were determined with real-time polymerase chain reaction (real-time PCR).
The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining.
The expression of TNF-α, IFN-γ, IL2, IL6, caspase 3, RIP1, beclin-1, and LC3 protein was assessed by immunofluorescence and western blotting.
Autophagosomes were observed with transmission electron microscopy (TEM).
Results.
Amelioration in liver functions and histopathological changes and the suppression of inflammatory cytokine production were observed in Nec-1-injected mice.
Western blotting analysis showed that the expression of TNF-α, IFN-γ, IL2, IL6, and RIP1 was significantly reduced in the Nec-1-injected mice, which was confirmed by immunofluorescence and immunohistochemistry.
Autophagosome formation was significantly reduced by Nec-1 treatment, as the expression of beclin-1 and LC3, determined with immunofluorescence and western blotting.
Conclusion.
These results demonstrate that Nec-1 prevents ConA-induced liver injury via RIP1-related and autophagy-related pathways.
American Psychological Association (APA)
Zhou, Ying-Qun& Weiqi, Dai& Lin, Chunlei& Wang, Fan& He, Lei& Shen, Miao…[et al.]. 2013. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-492072
Modern Language Association (MLA)
Zhou, Ying-Qun…[et al.]. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-492072
American Medical Association (AMA)
Zhou, Ying-Qun& Weiqi, Dai& Lin, Chunlei& Wang, Fan& He, Lei& Shen, Miao…[et al.]. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-492072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492072