Toxic Damage Increases Angiogenesis and Metastasis in Fibrotic Livers via PECAM-1
Joint Authors
Van Cayzeele, Christina Jay
Strassburg, Christian P.
Sauerbruch, Tilman
Raskopf, Esther
Gonzalez Carmona, Maria Angeles
Schmitz, Volker
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Excessive ethanol consumption is one of the main causes of liver fibrosis.
However, direct effects of ethanol exposure on endothelial cells and their contribution to fibrogenesis and metastasis were not investigated.
Therefore we analysed whether ethanol directly affects endothelial cells and if this plays a role during fibrogenesis and metastasis in the liver.
Murine and human endothelial cells were exposed to ethanol for up to 72 hours.
In vitro, effects on VEGF, HIF-1alpha, PECAM-1, and endothelial cell functions were analysed.
In vivo, effects of continuous liver damage on blood vessel formation and metastasis were analysed by PECAM-1 immunohistochemistry.
Ethanol increased HIF-1alpha and VEGF levels in murine and human endothelial cells.
This resulted in enhanced intracellular signal transduction, and PECAM-1 expression as well as tube formation and wound healing.
In vivo, toxic liver damage increased angiogenesis during fibrogenesis.
Metastasis was also enhanced in fibrotic livers and located to PECAM-1 positive blood vessels compared to nonfibrotic mice.
In conclusion, ethanol had strong effects on endothelial cells, which—at least in part—led to a profibrotic and prometastatic environment mediated by PECAM-1.
Blockade of increased PECAM-1 expression could be a promising tool to inhibit fibrogenesis and metastasis in the liver.
American Psychological Association (APA)
Raskopf, Esther& Gonzalez Carmona, Maria Angeles& Van Cayzeele, Christina Jay& Strassburg, Christian P.& Sauerbruch, Tilman& Schmitz, Volker. 2014. Toxic Damage Increases Angiogenesis and Metastasis in Fibrotic Livers via PECAM-1. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492583
Modern Language Association (MLA)
Raskopf, Esther…[et al.]. Toxic Damage Increases Angiogenesis and Metastasis in Fibrotic Livers via PECAM-1. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-492583
American Medical Association (AMA)
Raskopf, Esther& Gonzalez Carmona, Maria Angeles& Van Cayzeele, Christina Jay& Strassburg, Christian P.& Sauerbruch, Tilman& Schmitz, Volker. Toxic Damage Increases Angiogenesis and Metastasis in Fibrotic Livers via PECAM-1. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-492583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492583