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Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter
Joint Authors
Arbuthnot, Patrick
Mowa, Mohube Betty
Crowther, Carol
Ely, Abdullah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Research on applying RNA interference (RNAi) to counter HBV replication has led to identification of potential therapeutic sequences.
However, before clinical application liver-specific expression and efficient delivery of these sequences remain an important objective.
We recently reported short-term inhibition of HBV replication in vivo by using helper dependent adenoviral vectors (HD Ads) expressing anti-HBV sequences from a constitutively active cytomegalovirus (CMV) promoter.
To develop the use of liver-specific transcription regulatory elements we investigated the utility of the murine transthyretin (MTTR) promoter for expression of anti-HBV primary microRNAs (pri-miRs).
HD Ads containing MTTR promoter effected superior expression of anti-HBV pri-miRs in mice compared to HD Ads containing the CMV promoter.
MTTR-containing HD Ads resulted in HBV replication knockdown of up to 94% in mice.
HD Ads expressing trimeric anti-HBV pri-miRs silenced HBV replication for 5 weeks.
We previously showed that the product of the codelivered lacZ gene induces an immune response, and the duration of HBV silencing in vivo is likely to be attenuated by this effect.
Nevertheless, expression of anti-HBV pri-miRs from MTTR promoter is well suited to countering HBV replication and development of HD Ads through attenuation of their immunostimulatory effects should advance their clinical utility.
American Psychological Association (APA)
Mowa, Mohube Betty& Crowther, Carol& Ely, Abdullah& Arbuthnot, Patrick. 2014. Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-493109
Modern Language Association (MLA)
Mowa, Mohube Betty…[et al.]. Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-493109
American Medical Association (AMA)
Mowa, Mohube Betty& Crowther, Carol& Ely, Abdullah& Arbuthnot, Patrick. Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-493109
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493109