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Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide
Joint Authors
Chun, Byung-Soo
Kishimura, Hideki
Nalinanon, Sitthipong
Klomklao, Sappasith
Benjakul, Soottawat
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Viscera of mackerel (Scomber sp.) were defatted by supercritical carbon dioxide (SCO2) treatment.
Trypsin (SC-T) was then extracted from the defatted powder and purified by a series of chromatographies including Sephacryl S-200 and Sephadex G-50.
The purified SC-T was nearly homogeneous on SDS-PAGE, and its molecular weight was estimated as approximately 24,000 Da.
N-terminal twenty amino acids sequence of SC-T was IVGGYECTAHSQPHQVSLNS.
The specific trypsin inhibitors, soybean trypsin inhibitor and TLCK, strongly inhibited the activities of SC-T.
The pH and temperature optimums of SC-T were at around pH 8.0 and 60∘C, respectively, using Nα-p-tosyl-L-arginine methyl ester as a substrate.
The SC-T was unstable below pH 5.0 and above 40∘C, and it was stabilized by calcium ion.
These enzymatic characteristics of SC-T were the same as those of other fish trypsins, especially spotted mackerel (S.
borealis) trypsin, purified from viscera defatted by acetone.
Therefore, we concluded that the SCO2 defatting process is useful as a substitute for organic solvent defatting process.
American Psychological Association (APA)
Chun, Byung-Soo& Kishimura, Hideki& Nalinanon, Sitthipong& Klomklao, Sappasith& Benjakul, Soottawat. 2011. Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide. Journal of Amino Acids،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-493886
Modern Language Association (MLA)
Chun, Byung-Soo…[et al.]. Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide. Journal of Amino Acids No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-493886
American Medical Association (AMA)
Chun, Byung-Soo& Kishimura, Hideki& Nalinanon, Sitthipong& Klomklao, Sappasith& Benjakul, Soottawat. Mackerel Trypsin Purified from Defatted Viscera by Supercritical Carbon Dioxide. Journal of Amino Acids. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-493886
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493886