JNK12 Activation by an Extract from the Roots of Morus alba L. Reduces the Viability of Multidrug-Resistant MCF-7Dox Cells by Inhibiting YB-1-Dependent MDR1 Expression
Joint Authors
Woo, Sang-Mi
Ko, Seong-Gyu
Shin, Yong Cheol
Cho, Sung-Gook
Choi, Youn Kyung
Choi, Hyeong Sim
Yun, Yee Jin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cancer cells acquire anticancer drug resistance during chemotherapy, which aggravates cancer disease.
MDR1 encoded from multidrug resistance gene 1 mainly causes multidrug resistance phenotypes of different cancer cells.
In this study, we demonstrate that JNK1/2 activation by an extract from the root of Morus alba L.
(White mulberry) reduces doxorubicin-resistant MCF-7/Dox cell viability by inhibiting YB-1 regulation of MDR1 gene expression.
When MCF-7 or MCF-7/Dox cells, where MDR1 is highly expressed were treated with an extract from roots or leaves of Morus alba L., respectively, the root extract from the mulberry (REM) but not the leaf extract (LEM) reduced cell viabilities of both MCF-7 and MCF-7/Dox cells, which was enhanced by cotreatment with doxorubicin.
REM but not LEM further inhibited YB-1 nuclear translocation and its regulation of MDR1 gene expression.
Moreover, REM promoted phosphorylation of c-Jun NH2-terminal kinase 1/2 (JNK1/2) and JNK1/2 inhibitor, SP600125 and rescued REM inhibition of both MDR1 expression and viabilities in MCF-7/Dox cells.
Consistently, overexpression of JNK1, c-Jun, or c-Fos inhibited YB-1-dependent MDR1 expression and reduced viabilities in MCF-7/Dox cells.
In conclusion, our data indicate that REM-activated JNK-cJun/c-Fos pathway decreases the viability of MCF-7/Dox cells by inhibiting YB-1-dependent MDR1 gene expression.
Thus, we suggest that REM may be useful for treating multidrug-resistant cancer cells.
American Psychological Association (APA)
Choi, Youn Kyung& Cho, Sung-Gook& Choi, Hyeong Sim& Woo, Sang-Mi& Yun, Yee Jin& Shin, Yong Cheol…[et al.]. 2013. JNK12 Activation by an Extract from the Roots of Morus alba L. Reduces the Viability of Multidrug-Resistant MCF-7Dox Cells by Inhibiting YB-1-Dependent MDR1 Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495103
Modern Language Association (MLA)
Choi, Youn Kyung…[et al.]. JNK12 Activation by an Extract from the Roots of Morus alba L. Reduces the Viability of Multidrug-Resistant MCF-7Dox Cells by Inhibiting YB-1-Dependent MDR1 Expression. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-495103
American Medical Association (AMA)
Choi, Youn Kyung& Cho, Sung-Gook& Choi, Hyeong Sim& Woo, Sang-Mi& Yun, Yee Jin& Shin, Yong Cheol…[et al.]. JNK12 Activation by an Extract from the Roots of Morus alba L. Reduces the Viability of Multidrug-Resistant MCF-7Dox Cells by Inhibiting YB-1-Dependent MDR1 Expression. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495103
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495103