Hepatic Expression of Metallothionein III, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis
Joint Authors
Grubić-Kezele, Tanja
Blagojević Zagorac, Gordana
Jakovac, Hrvoje
Radošević-Stašić, Biserka
Domitrović, Robert
Milin, Čedomila
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In a search of peripheral factors that could be responsible for the discrepancy in susceptibility to EAE in Albino Oxford (AO) and Dark Agouti (DA) rats, we estimated the expression of metallothioneins I/II (MT), heat shock protein-gp96, interleukin (IL)-6, and transforming growth factor (TGF)-β in the livers of these animals.
Rats were immunized with bovine brain homogenate (BBH) emulsified in complete Freund adjuvant (CFA) or only with CFA.
Western blot and immunohistochemical analyses were done on day 12 after the immunization, as well as in intact rats.
The data have shown that during the first attack of EAE only the EAE prone-DA rats markedly upregulated the hepatic MTs, gp96, IL-6, and TGF-β.
In contrast, AO rats had a significantly higher expression of MT I/II, IL-6, and TGF-β in intact liver (P<0,001), suggesting that the greater constitutive expression of these proteins contributed to the resistance of EAE.
Besides, since previously we found that AO rats reacted on immunization by an early upregulation of TGF-β on several hepatic structures (vascular endothelium, Kupffer cells, and hepatocytes), the data suggest that the specific hepatic microenvironment might contribute also to the faster recovery of these rats from EAE.
American Psychological Association (APA)
Grubić-Kezele, Tanja& Blagojević Zagorac, Gordana& Jakovac, Hrvoje& Domitrović, Robert& Milin, Čedomila& Radošević-Stašić, Biserka. 2013. Hepatic Expression of Metallothionein III, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495768
Modern Language Association (MLA)
Grubić-Kezele, Tanja…[et al.]. Hepatic Expression of Metallothionein III, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis. Clinical and Developmental Immunology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-495768
American Medical Association (AMA)
Grubić-Kezele, Tanja& Blagojević Zagorac, Gordana& Jakovac, Hrvoje& Domitrović, Robert& Milin, Čedomila& Radošević-Stašić, Biserka. Hepatic Expression of Metallothionein III, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495768