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Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant
Joint Authors
Chen, Der-Yuan
Ko, Ching-Huai
Lin, Sheng-Hao
Lin, Ching-Hsiung
Lue, Yun-Sheng
Chu, Ching-Liang
Lin, Chi-Chen
Chen, Mei-Hsing
Li, Wei-Sung
Chang, Chih-Peng
Pan, I-Horng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-22
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
This work represents the first evaluation of the effects of water extract of C.
nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated.
Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules.
In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells.
Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN.
Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-κB p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2.
Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth.
These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy.
American Psychological Association (APA)
Chen, Mei-Hsing& Li, Wei-Sung& Lue, Yun-Sheng& Chu, Ching-Liang& Pan, I-Horng& Ko, Ching-Huai…[et al.]. 2013. Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-496699
Modern Language Association (MLA)
Chen, Mei-Hsing…[et al.]. Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-496699
American Medical Association (AMA)
Chen, Mei-Hsing& Li, Wei-Sung& Lue, Yun-Sheng& Chu, Ching-Liang& Pan, I-Horng& Ko, Ching-Huai…[et al.]. Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-496699
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496699