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Using the Doubly Charged Selected Ion Coupled with MSMS Fragments Monitoring (DCSI-MSMS) Mode for the Identification of Gelatin Species
Joint Authors
Ma, Shuang-cheng
Zhang, Lei
Li, Ming-hua
Xiao, Xin-Yue
Zhao, Ying-Yong
Lin, Rui-Chao
Chen, Jia
Cheng, Xian-Long
Wei, Feng
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In electrospray ionization (ESI) mode, peptides and proteins can be multiply charged ions; in this situation a doubly charged selected ion (DCSI) coupled with mass spectrometry (MS/MS) fragments monitoring (DCSI-MS/MS) method is the most suitable scanning mode to detect known peptides in complex samples when an ion-trap mass spectrometer is the instrument used for the analysis.
In this mode, the MS detector is programmed to only select a doubly charged ion as a precursor and to perform continuous MS/MS on one or more of the selected precursors, either during a specific time interval or along the whole chromatographic run.
Gelatin is a mixture of high molecular weight polypeptides from the hydrolysis of collagen.
In this study, the DCSI-MS/MS monitoring mode was applied to the detection of previously characterized species-specific peptides from different gelatins.
The proposed methodology makes use of tryptic digestion for sample preparation and peptide separation and identification by rapid resolution liquid chromatography coupled to an ion trap working in the DCSI-MS/MS mode for the analysis.
This methodology was applied to the differential classification of five commercial, homological species of gelatins and proved to be an excellent tool for gelatin product authentication.
American Psychological Association (APA)
Cheng, Xian-Long& Wei, Feng& Chen, Jia& Li, Ming-hua& Zhang, Lei& Zhao, Ying-Yong…[et al.]. 2014. Using the Doubly Charged Selected Ion Coupled with MSMS Fragments Monitoring (DCSI-MSMS) Mode for the Identification of Gelatin Species. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-496984
Modern Language Association (MLA)
Cheng, Xian-Long…[et al.]. Using the Doubly Charged Selected Ion Coupled with MSMS Fragments Monitoring (DCSI-MSMS) Mode for the Identification of Gelatin Species. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-496984
American Medical Association (AMA)
Cheng, Xian-Long& Wei, Feng& Chen, Jia& Li, Ming-hua& Zhang, Lei& Zhao, Ying-Yong…[et al.]. Using the Doubly Charged Selected Ion Coupled with MSMS Fragments Monitoring (DCSI-MSMS) Mode for the Identification of Gelatin Species. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-496984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496984