Catalytical Properties of Free and Immobilized Aspergillus niger Tannase
Joint Authors
Rodríguez-Durán, Luis V.
Aguilar, Cristóbal N.
Flores-Maltos, Abril
Renovato, Jacqueline
Contreras, Juan C.
Rodríguez, Raúl
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A fungal tannase was produced, recovered, and immobilized by entrapment in calcium alginate beads.
Catalytical properties of the immobilized enzyme were compared with those of the free one.
Tannase was produced intracellularly by the xerophilic fungus Aspergillus niger GH1 in a submerged fermentation system.
Enzyme was recovered by cell disruption and the crude extract was partially purified.
The catalytical properties of free and immobilized tannase were evaluated using tannic acid and methyl gallate as substrates.
KM and Vmax values for free enzyme were very similar for both substrates.
But, after immobilization, KM and Vmax values increased drastically using tannic acid as substrate.
These results indicated that immobilized tannase is a better biocatalyst than free enzyme for applications on liquid systems with high tannin content, such as bioremediation of tannery or olive-mill wastewater.
American Psychological Association (APA)
Flores-Maltos, Abril& Rodríguez-Durán, Luis V.& Renovato, Jacqueline& Contreras, Juan C.& Rodríguez, Raúl& Aguilar, Cristóbal N.. 2011. Catalytical Properties of Free and Immobilized Aspergillus niger Tannase. Enzyme Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-497217
Modern Language Association (MLA)
Flores-Maltos, Abril…[et al.]. Catalytical Properties of Free and Immobilized Aspergillus niger Tannase. Enzyme Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-497217
American Medical Association (AMA)
Flores-Maltos, Abril& Rodríguez-Durán, Luis V.& Renovato, Jacqueline& Contreras, Juan C.& Rodríguez, Raúl& Aguilar, Cristóbal N.. Catalytical Properties of Free and Immobilized Aspergillus niger Tannase. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-497217
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497217