![](/images/graphics-bg.png)
Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia
Joint Authors
Schemmer, Peter
Schneider, Heinz
Yi, Xue
Mohr, Elvira
Liang, Rui
Büchler, Markus W.
Mikalauskas, Saulius
Gross, Marie-Luise
Benzing, Steffen
Zorn, Markus
Li, Zhanqing
Nickkholgh, Arash
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Several approaches have been proposed to pharmacologically ameliorate hepatic ischemia/reperfusion injury (IRI).
This study was designed to evaluate the effects of a preconditioning oral nutritional supplement (pONS) containing glutamine, antioxidants, and green tea extract on hepatic warm IRI in pigs.
Methods.
pONS (70 g per serving, Fresenius Kabi, Germany) was dissolved in 250 mL tap water and given to pigs 24, 12, and 2 hrs before warm ischemia of the liver.
A fourth dose was given 3 hrs after reperfusion.
Controls were given the same amount of cellulose with the same volume of water.
Two hours after the third dose of pONS, both the portal vein and the hepatic artery were clamped for 40 min.
0.5, 3, 6, and 8 hrs after reperfusion, heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), portal venous flow (PVF), hepatic arterial flow (HAF), bile flow, and transaminases were measured.
Liver tissue was taken 8 hrs after reperfusion for histology and immunohistochemistry.
Results.
HR, MAP, CVP, HAF, and PVF were comparable between the two groups.
pONS significantly increased bile flow 8 hrs after reperfusion.
ALT and AST were significantly lower after pONS.
Histology showed significantly more severe necrosis and neutrophil infiltration in controls.
pONS significantly decreased the index of immunohistochemical expression for TNF-α, MPO, and cleaved caspase-3 (P<0.001).
Conclusion.
Administration of pONS before and after tissue damage protects the liver from warm IRI via mechanisms including decreasing oxidative stress, lipid peroxidation, apoptosis, and necrosis.
American Psychological Association (APA)
Nickkholgh, Arash& Li, Zhanqing& Yi, Xue& Mohr, Elvira& Liang, Rui& Mikalauskas, Saulius…[et al.]. 2012. Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia. HPB Surgery،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-497725
Modern Language Association (MLA)
Nickkholgh, Arash…[et al.]. Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia. HPB Surgery No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-497725
American Medical Association (AMA)
Nickkholgh, Arash& Li, Zhanqing& Yi, Xue& Mohr, Elvira& Liang, Rui& Mikalauskas, Saulius…[et al.]. Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia. HPB Surgery. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-497725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497725