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Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal StemProgenitor Cells
Joint Authors
Zhang, Ping
Zhang, Wenxiu
Guan, Weijun
He, Xiaohong
Li, Changli
Ma, Yuehui
Li, Xiangchen
Pu, Yabin
Cui, Peng
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were a multipotential stem cell population, which has potential applications in the tissue damage repair and skin transplant.
Although a large number of studies deal with the human DMS/PCs self-renewal and regulation, however, the study of livestock-derived DMS/PCs has rarely been reported.
Here, sheep DMS/PCs were isolated from one-month-old sheep embryos and studied at the cellular and molecular level.
And then the DMS/PCs biological characteristics were analysed by RT-PCR and immunofluorescence.
Experimental results showed that DMS/PCs could be expanded for 48 passages and the cells viability and hereditary character were steady.
In addition, the DMS/PCs maker β-integrin, CD71, CD44, and CD73 were expressed positively through RT-PCR and immunofluorescence.
Passage 3 DMS/PCs were successfully induced to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes, respectively.
The above results suggest that DMS/PCs not only have strong self-renewal capacity but also have the potential to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes.
The study provides theoretical basis and experimental evidence for potential clinical application.
American Psychological Association (APA)
Cui, Peng& He, Xiaohong& Pu, Yabin& Zhang, Wenxiu& Zhang, Ping& Li, Changli…[et al.]. 2014. Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal StemProgenitor Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-497985
Modern Language Association (MLA)
Cui, Peng…[et al.]. Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal StemProgenitor Cells. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-497985
American Medical Association (AMA)
Cui, Peng& He, Xiaohong& Pu, Yabin& Zhang, Wenxiu& Zhang, Ping& Li, Changli…[et al.]. Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal StemProgenitor Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-497985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497985