Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model
Joint Authors
Dixon, Giles
Whyte, Moira K. B.
Billings, Stephen A.
Holmes, Geoffrey R.
Reyes-Aldasoro, Constantino Carlos
Elks, Philip M.
Renshaw, Stephen A.
Kadirkamanathan, Visakan
Anderson, Sean R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Neutrophils must be removed from inflammatory sites for inflammation to resolve.
Recent work in zebrafish has shown neutrophils can migrate away from inflammatory sites, as well as die in situ.
The signals regulating the process of reverse migration are of considerable interest, but remain unknown.
We wished to study the behaviour of neutrophils during reverse migration, to see whether they moved away from inflamed sites in a directed fashion in the same way as they are recruited or whether the inherent random component of their migration was enough to account for this behaviour.
Using neutrophil-driven photoconvertible Kaede protein in transgenic zebrafish larvae, we were able to specifically label neutrophils at an inflammatory site generated by tailfin transection.
The locations of these neutrophils over time were observed and fitted using regression methods with two separate models: pure-diffusion and drift-diffusion equations.
While a model hypothesis test (the F-test) suggested that the datapoints could be fitted by the drift-diffusion model, implying a fugetaxis process, dynamic simulation of the models suggested that migration of neutrophils away from a wound is better described by a zero-drift, “diffusion” process.
This has implications for understanding the mechanisms of reverse migration and, by extension, neutrophil retention at inflammatory sites.
American Psychological Association (APA)
Holmes, Geoffrey R.& Dixon, Giles& Anderson, Sean R.& Reyes-Aldasoro, Constantino Carlos& Elks, Philip M.& Billings, Stephen A.…[et al.]. 2012. Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model. Advances in Hematology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498471
Modern Language Association (MLA)
Holmes, Geoffrey R.…[et al.]. Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model. Advances in Hematology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-498471
American Medical Association (AMA)
Holmes, Geoffrey R.& Dixon, Giles& Anderson, Sean R.& Reyes-Aldasoro, Constantino Carlos& Elks, Philip M.& Billings, Stephen A.…[et al.]. Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model. Advances in Hematology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498471