Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization
Joint Authors
Alves Macedo, Gabriela
Ries, Edi Franciele
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium.
Concentrations of carbon source and inducer of phytase production were optimized using a 22 full factorial CCD and response surface methodology (RSM).
Urea was fixed as nitrogen source in culture medium (0.15%, w/v).
The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity.
In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production.
The validity of this model was verified by culturing the organisms in flasks on a shaker.
Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S.
cerevisiae.
American Psychological Association (APA)
Ries, Edi Franciele& Alves Macedo, Gabriela. 2011. Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization. Enzyme Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-498855
Modern Language Association (MLA)
Ries, Edi Franciele& Alves Macedo, Gabriela. Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization. Enzyme Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-498855
American Medical Association (AMA)
Ries, Edi Franciele& Alves Macedo, Gabriela. Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-498855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498855