Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes
Joint Authors
Upadhyaya, Supriya
Ramteke, Pramod
Joseph, Babu
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues.
In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas.
Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lipases at 15 ± 1°C within 48 h.
Supplementation of glucose 1% (w/v) as additional carbon source and ammonium nitrate 2% (w/v) as additional nitrogen source enhanced production of lipase.
Addition of triglycerides 0.5% (v/v) tends to repress the lipase production.
Further mixed preparation of groundnut oil cake (GOC) along with mustard oil cake (MOC) in the ratio of 1 : 1, and its optimization resulted in improved production of cold active lipase.
The enzyme exhibited maximum activity at 10–15°C and was stable at temperatures lower than 30°C.
The lipase exhibited optimum activity at pH 8 and showed more than 60% stability at pH 9.
Semisolid state fermentation process by utilizing agroindustrial wastes will direct to large-scale commercialization of lipase catalyzed process in cost-effective systems.
American Psychological Association (APA)
Joseph, Babu& Upadhyaya, Supriya& Ramteke, Pramod. 2011. Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes. Enzyme Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-498858
Modern Language Association (MLA)
Joseph, Babu…[et al.]. Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes. Enzyme Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-498858
American Medical Association (AMA)
Joseph, Babu& Upadhyaya, Supriya& Ramteke, Pramod. Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-498858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498858