BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK12 Signaling Pathway
Joint Authors
Liang, Rixin
Wang, Lan
Yang, Hongjun
Zhang, Fangbo
Tang, Shihuan
Xu, Haiyu
Zhao, Ye
Huang, Bin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Buchang naoxintong capsule (BNC) is a traditional Chinese medicine approved for the treatment of cerebrovascular and cardiovascular diseases.
However, little is known about the specific protective function or mechanism by which BNC protects against myocardial injury.
This research was designed to investigate the cardioprotective effects of BNC in vitro model of hydrogen peroxide (H2O2)-induced H9c2 rat cardiomyoblasts.
BNC intestinal absorption liquid was used in this study instead of drug-containing serum or extracting solution.
Our study revealed that BNC preconditioning enhanced antioxidant function by increasing the activities of total-antioxygen capacity, total-superoxide dismutase, and catalase and by decreasing the production of reactive oxygen species and malondialdehyde.
BNC preconditioning also activated extracellular signal-regulated kinases (ERK1/2) and inhibited apoptosis-related proteins such as poly ADP-ribose polymerase (PARP) and caspase-3.
Additionally, preincubation with BNC reduced intracellular Ca2+ concentration, improved mitochondrial membrane potential, and decreased the apoptosis rate of H9c2 cells in a dose-dependent manner.
These data demonstrated that BNC protects H9c2 cardiomyoblasts from H2O2-induced oxidative injury by increasing antioxidant abilities, activating ERK1/2, and blocking Ca2+-dependent and mitochondria-mediated apoptosis.
Based on our results, the potency of BNC for protecting H9c2 cells from oxidative damage is comparable to that of trimetazidine.
American Psychological Association (APA)
Zhang, Fangbo& Huang, Bin& Zhao, Ye& Tang, Shihuan& Xu, Haiyu& Wang, Lan…[et al.]. 2013. BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-499240
Modern Language Association (MLA)
Zhang, Fangbo…[et al.]. BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-499240
American Medical Association (AMA)
Zhang, Fangbo& Huang, Bin& Zhao, Ye& Tang, Shihuan& Xu, Haiyu& Wang, Lan…[et al.]. BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-499240
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499240