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Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions
Joint Authors
Fernandes Pimentel, Maria Ines
de Freitas Campos Miranda, Luciana
Madeira, Maria de Fátima
Conceição-Silva, Fatima
Helena da Silva Barros, Juliana
Schubach, Armando de Oliveira
Baptista, Cibele
da Silva Pacheco, Raquel
Guimarães de Souza Pinto, Andressa
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The aim of this study was to investigate genetic polymorphism in Leishmania braziliensis population previously typed through isoenzyme electrophoresis, isolated from the same patient in two different moments: (A) before the beginning of treatment and (B) after treatment failure to meglumine antimoniate or reactivation after successful initial treatment.
Fifteen pairs of isolates were assessed using the polymorphic molecular marker LSSP-PCR and following the phenetic analysis.
The genetic profiles of the 30 samples were grouped in four clusters.
Only two patients presented total identity in the A and B isolates.
Most isolates presented similarity coefficients varying from 0.63 to 0.91.
In this group of patients genetic polymorphisms could be observed indicating low similarity between the pairs of isolates.
The results demonstrate the existence of genetic polymorphism between the samples isolated before treatment and after reactivation or treatment failure, suggesting a possible differentiation of the structure of the original parasite population which could be involved in the mechanisms of resistance to treatment or reactivation of lesions in the ATL.
This phenomenon is important, although other factors also could be involved in this context and are discussed in this paper.
American Psychological Association (APA)
Baptista, Cibele& Schubach, Armando de Oliveira& Madeira, Maria de Fátima& de Freitas Campos Miranda, Luciana& Guimarães de Souza Pinto, Andressa& Helena da Silva Barros, Juliana…[et al.]. 2012. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-499714
Modern Language Association (MLA)
Baptista, Cibele…[et al.]. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-499714
American Medical Association (AMA)
Baptista, Cibele& Schubach, Armando de Oliveira& Madeira, Maria de Fátima& de Freitas Campos Miranda, Luciana& Guimarães de Souza Pinto, Andressa& Helena da Silva Barros, Juliana…[et al.]. Evaluation of Genetic Polymorphism of Leishmania (V.) braziliensis Isolates Obtained from the Same Patient before and after Therapeutic Failure or Reactivation of Cutaneous Lesions. Journal of Tropical Medicine. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-499714
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499714