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New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage
Joint Authors
Crovella, Sérgio
Ferreira Neto, José Ribamar Costa
Benko-Iseppon, Ana Maria
Guimarães, Ana Carolina Ribeiro
Silva, Roberta Lane de Oliveira
Kido, Éderson Akio
Pandolfi, Valesca
Chabregas, Sabrina Moutinho
Veiga, Daniela Truffi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
In the scope of the present work, four SuperSAGE libraries have been generated, using bulked root tissues from four drought-tolerant accessions as compared with four bulked sensitive genotypes, aiming to generate a panel of differentially expressed stress-responsive genes.
Both groups were submitted to 24 hours of water deficit stress.
The SuperSAGE libraries produced 8,787,315 tags (26 bp) that, after exclusion of singlets, allowed the identification of 205,975 unitags.
Most relevant BlastN matches comprised 567,420 tags, regarding 75,404 unitags with 164,860 different ESTs.
To optimize the annotation efficiency, the Gene Ontology (GO) categorization was carried out for 186,191 ESTs (BlastN against Uniprot-SwissProt), permitting the categorization of 118,208 ESTs (63.5%).
In an attempt to elect a group of the best tags to be validated by RTqPCR, the GO categorization of the tag-related ESTs allowed the in silico identification of 213 upregulated unitags responding basically to abiotic stresses, from which 145 presented no hits after BlastN analysis, probably concerning new genes still uncovered in previous studies.
The present report analyzes the sugarcane transcriptome under drought stress, using a combination of high-throughput transcriptome profiling by SuperSAGE with the Solexa sequencing technology, allowing the identification of potential target genes during the stress response.
American Psychological Association (APA)
Kido, Éderson Akio& Ferreira Neto, José Ribamar Costa& Silva, Roberta Lane de Oliveira& Pandolfi, Valesca& Guimarães, Ana Carolina Ribeiro& Veiga, Daniela Truffi…[et al.]. 2012. New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-500814
Modern Language Association (MLA)
Kido, Éderson Akio…[et al.]. New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage. The Scientific World Journal No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-500814
American Medical Association (AMA)
Kido, Éderson Akio& Ferreira Neto, José Ribamar Costa& Silva, Roberta Lane de Oliveira& Pandolfi, Valesca& Guimarães, Ana Carolina Ribeiro& Veiga, Daniela Truffi…[et al.]. New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-500814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500814