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ProBLM Web Server : Protein and Membrane Placement and Orientation Package
Joint Authors
Sarkar, Subhra
Petukh, Marharyta
Alexov, Emil
Kimmett, Taylor
Witham, Shawn
Smith, Nicholas
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The 3D structures of membrane proteins are typically determined without the presence of a lipid bilayer.
For the purpose of studying the role of membranes on the wild type characteristics of the corresponding protein, determining the position and orientation of transmembrane proteins within a membrane environment is highly desirable.
Here we report a geometry-based approach to automatically insert a membrane protein with a known 3D structure into pregenerated lipid bilayer membranes with various dimensions and lipid compositions or into a pseudomembrane.
The pseudomembrane is built using the Protein Nano-Object Integrator which generates a parallelepiped of user-specified dimensions made up of pseudoatoms.
The pseudomembrane allows for modeling the desolvation effects while avoiding plausible errors associated with wrongly assigned protein-lipid contacts.
The method is implemented into a web server, the ProBLM server, which is freely available to the biophysical community.
The web server allows the user to upload a protein coordinate file and any missing residues or heavy atoms are regenerated.
ProBLM then creates a combined protein-membrane complex from the given membrane protein and bilayer lipid membrane or pseudomembrane.
The user is given an option to manually refine the model by manipulating the position and orientation of the protein with respect to the membrane.
American Psychological Association (APA)
Kimmett, Taylor& Smith, Nicholas& Witham, Shawn& Petukh, Marharyta& Sarkar, Subhra& Alexov, Emil. 2014. ProBLM Web Server : Protein and Membrane Placement and Orientation Package. Computational and Mathematical Methods in Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-502192
Modern Language Association (MLA)
Kimmett, Taylor…[et al.]. ProBLM Web Server : Protein and Membrane Placement and Orientation Package. Computational and Mathematical Methods in Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-502192
American Medical Association (AMA)
Kimmett, Taylor& Smith, Nicholas& Witham, Shawn& Petukh, Marharyta& Sarkar, Subhra& Alexov, Emil. ProBLM Web Server : Protein and Membrane Placement and Orientation Package. Computational and Mathematical Methods in Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-502192
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502192