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The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL6J Mice
Joint Authors
Perrard, Xiaoyuan Dai
Raya, Joe
Gaubatz, John
Hoogeveen, Ron C.
Wu, Huaizhu
Wooten, Joshua S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Our current understanding of oxysterol metabolism during different disease states such as obesity and dyslipidemia is limited.
Therefore, the aim of this study was to determine the effect of diet-induced obesity on the tissue distribution of various oxysterols and the mRNA expression of key enzymes involved in oxysterol metabolism.
To induce obesity, male C57BL/6J mice were fed a high fat-cholesterol diet for 24 weeks.
Following diet-induced obesity, plasma levels of 4β-hydroxycholesterol, 5,6α-epoxycholesterol, 5,6β-epoxycholesterol, 7α-hydroxycholesterol, 7β-hydroxycholesterol, and 27-hydroxycholesterol were significantly (P<0.05) increased.
In the liver and adipose tissue of the obese mice, 4β-hydroxycholesterol was significantly (P<0.05) increased, whereas 27-hydroxycholesterol was increased only in the adipose tissue.
No significant changes in either hepatic or adipose tissue mRNA expression were observed for oxysterol synthesizing enzymes 4β-hydroxylase, 27-hydroxylase, or 7α-hydroxylase.
Hepatic mRNA expression of SULT2B1b, a key enzyme involved in oxysterol detoxification, was significantly (P<0.05) elevated in the obese mice.
Interestingly, the appearance of the large HDL1 lipoprotein was observed with increased oxysterol synthesis during obesity.
In diet-induced obese mice, dietary intake and endogenous enzymatic synthesis of oxysterols could not account for the increased oxysterol levels, suggesting that nonenzymatic cholesterol oxidation pathways may be responsible for the changes in oxysterol metabolism.
American Psychological Association (APA)
Wooten, Joshua S.& Wu, Huaizhu& Raya, Joe& Perrard, Xiaoyuan Dai& Gaubatz, John& Hoogeveen, Ron C.. 2014. The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL6J Mice. Cholesterol،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-502712
Modern Language Association (MLA)
Wooten, Joshua S.…[et al.]. The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL6J Mice. Cholesterol No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-502712
American Medical Association (AMA)
Wooten, Joshua S.& Wu, Huaizhu& Raya, Joe& Perrard, Xiaoyuan Dai& Gaubatz, John& Hoogeveen, Ron C.. The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL6J Mice. Cholesterol. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-502712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502712