In Silico Characterization of Histidine Acid Phytase Sequences
Joint Authors
Verma, A. K.
Kumar, Vinod
Agrawal, Sanjeev
Singh, Gopal
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Histidine acid phytases (HAPhy) are widely distributed enzymes among bacteria, fungi, plants, and some animal tissues.
They have a significant role as an animal feed enzyme and in the solubilization of insoluble phosphates and minerals present in the form of phytic acid complex.
A set of 50 reference protein sequences representing HAPhy were retrieved from NCBI protein database and characterized for various biochemical properties, multiple sequence alignment (MSA), homology search, phylogenetic analysis, motifs, and superfamily search.
MSA using MEGA5 revealed the presence of conserved sequences at N-terminal “RHGXRXP” and C-terminal “HD.” Phylogenetic tree analysis indicates the presence of three clusters representing different HAPhy, that is, PhyA, PhyB, and AppA.
Analysis of 10 commonly distributed motifs in the sequences indicates the presence of signature sequence for each class.
Motif 1 “SPFCDLFTHEEWIQYDYLQSLGKYYGYGAGNPLGPAQGIGF” was present in 38 protein sequences representing clusters 1 (PhyA) and 2 (PhyB).
Cluster 3 (AppA) contains motif 9 “KKGCPQSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDP” as a signature sequence.
All sequences belong to histidine acid phosphatase family as resulted from superfamily search.
No conserved sequence representing 3- or 6-phytase could be identified using multiple sequence alignment.
This in silico analysis might contribute in the classification and future genetic engineering of this most diverse class of phytase.
American Psychological Association (APA)
Kumar, Vinod& Singh, Gopal& Verma, A. K.& Agrawal, Sanjeev. 2012. In Silico Characterization of Histidine Acid Phytase Sequences. Enzyme Research،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502807
Modern Language Association (MLA)
Kumar, Vinod…[et al.]. In Silico Characterization of Histidine Acid Phytase Sequences. Enzyme Research No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-502807
American Medical Association (AMA)
Kumar, Vinod& Singh, Gopal& Verma, A. K.& Agrawal, Sanjeev. In Silico Characterization of Histidine Acid Phytase Sequences. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-502807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502807